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The Skin Graft Rejection Against HLA-A2 Transgenic Mice is More Likely to be Acute Rejection

Author: YuZuo
Tutor: WuXiongWen
School: Huazhong University of Science and Technology
Course: Immunology
Keywords: Transgenic mice HLA-A2 Skin graft Mixed lymphocyte culture
CLC: R392
Type: Master's thesis
Year: 2009
Downloads: 9
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Abstract

Allogenic transplant rejection reaction identify the target antigen is expressed on the cell surface of the graft the major histocompatibility complex ( MHC ) molecules , due to several types of major histocompatibility complex (HLA) genes abundant polymorphic difficult few body level study single allogeneic HLA molecules in transplant rejection role . HLA. A2 transgenic mice appear ideal animal model for in vivo studies transplantation rejection mechanisms and interventions . The topics to C57BL / 6 the source of HLA - A2 transgenic mice (hereinafter referred to as the A2 mouse) as the major experimental object , the establishment of the skin caused by the only differences in the HLA-A2 gene transplant rejection animal models , and by comparing the HLA-A2 gene graft rejection caused by the difference in the reaction with typical acute rejection reaction and chronic rejection of the similarities and differences , and to explore this type of transplant rejection and mechanisms . The main contents and results of this study are as follows: 1 HLA-A2 -specific primers the RT-PcR and the HLA-A2 molecule specific antibodies BB7.2 detection of HLA-A2 transgenic mouse cell surface expression of HLA-A2 molecule . 2 ordinary c57BL / 6 male mice , male A2 mouse donor , only the skin caused by genetic differences in HLA-A2 transplant rejection in animal models , the results found for the the A2 mouse skin graft rejection occur rapidly , and 9 to 13 days , the skin graft rejection . Both spleen cells in the mixed lymphocyte culture, the results show the C57BL / 6 derived T cells after in vitro cell stimulation by A2 mice proliferated significantly . 3 with allogeneic skin grafts in mice caused by acute rejection reaction dioecious transplant chronic rejection compared for HLA-A2 transfected the gene mouse skin graft rejection and in vitro showed alloreactive occur rapid reaction and strength characteristics of acute rejection . Between donor and recipient , there is only a difference of HLA-A2 A2 mouse skin transplant rejection closer acute rejection , so the animal model can be used to further understanding of transplant rejection in direct recognition mechanism and explore the prevention and treatment of transplant rejection means.

The Skin Graft Rejection Against HLA-A2 Transgenic Mice is More Likely to be Acute Rejection

Abstract

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