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Study on Anti-angiogenesis Effects of Albizia Different Extraction Fractions

Author: LiuLingYan
Tutor: QiuLiYing
School: Jiangnan University
Course: Microbial and Biochemical Pharmacy
Keywords: Albizia Extraction Process Endothelial cells Toxicity Pharmacodynamic
CLC: R285
Type: Master's thesis
Year: 2010
Downloads: 77
Quote: 0
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Abstract


Tumor angiogenesis in tumor growth and metastasis of nutrition and ways to inhibit tumor angiogenesis is an important method for the treatment of tumors. At home and abroad have listed for tumor vascular drug (Avastin Endostar), but these drugs are expensive and can only be administered by injection, so the development of low-dosage oral anti-tumor vascular formation of the drug is a tumor therapy The endothelial cell is one of the objectives of the study, the most important cell in the cells involved in angiogenesis, inhibit the growth of endothelial cells will be able to prevent the formation of blood vessels and thereby play an inhibitory effect on the tumor. In this paper, human microvascular endothelial cells (HMEC-1) on a variety of traditional Chinese medicine Albizia extraction component model for drug screening, screening, and further through the active component of the dry matter yield, the ease of the process route, endothelial cell toxicity Evaluation, looking for efficiency and low toxicity of the active component, and effective component in vivo toxicology, pharmacodynamics in vitro and in vivo pharmacodynamic discussed. The paper identified three effective components extracted route, namely raw lyophilized powder aqueous solution, washed with petroleum ether, n-butanol saturated with water extraction, the resulting n-butanol phase (fraction 19); alcohol sedimentation alcohol The dissolved part of the aqueous solution of lyophilized powder with water saturated n-butanol extract, and the resulting n-butanol phase (component 21); ethanol reflux after the aqueous solution of the lyophilized powder with water saturated n-butanol extract, and the resulting n-butanol phase (component 23 ). Three line process is simple and high yield, dry matter, activity and toxicity. Three extracts in vitro pharmacodynamic study found a significant inhibition of the activity of the vascular endothelial cell proliferation. And the three extracts having significant inhibition of the activity of the vascular endothelial cell migration. Species and 23 with significant inhibition of angiogenesis. The three extracts toxicology studies found that toxic manifestations: prolonged blood clotting time; reduce the frequency of spontaneous activity; obvious damage to the heart, liver, kidney, lung, spleen; heartless brain lung and kidney tissue MDA content, lower SOD activity; varying degrees of elevated serum BUN, GOT and GPT concentration. By further comparison of the toxicity of three active components, the component 23 minimal toxicity for the best components, so to further investigate the in vivo efficacy. Component 23 in vivo pharmacodynamic studies have shown that compared with the model group, given the component 23 can improve the quality of life of tumor-bearing mice; able to inhibit the growth of tumors, Histological tumor cells appear karyopyknosis piece necrosis and the surrounding inflammatory cell infiltration, etc.;, and in a dose correlation. Established the Albizia herbs extraction rate, extract biological activity, toxicity for the extraction of the evaluation process, extract components inhibit vascular endothelial cell proliferation, Chinese medicine five categories of reporting generated to Albizia anti neovascularization effective part new drugs to create the conditions.

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