Dissertation > Excellent graduate degree dissertation topics show

The Structural Comparison of Angelica Sinensis Polysaccharide Before and After the Synthesis of APIC

Author: GuoDan
Tutor: WangKaiPing
School: Huazhong University of Science and Technology
Course: Pharmacy
Keywords: Angelica polysaccharide Separate Purification Molecular weight Monosaccharide composition Connection Glycosidic bond Sugar residue
CLC: R284.1
Type: Master's thesis
Year: 2009
Downloads: 74
Quote: 0
Read: Download Dissertation

Abstract


Angelica polysaccharide active ingredients the Chinese angelica play a role of blood and blood. The reference listed polysaccharide iron complex material resources Philippine energy, our laboratory to the of Angelica polysaccharide and ferric chloride as raw materials successfully synthesized Angelica polysaccharide iron complex pharmacological experiments confirmed that the the angelica polysaccharide iron complex is indeed significant role of iron, and can produce a synergistic role in the blood. This paper first reported the the angelica the polysaccharide ASP1 and ASP2 structure, and polysaccharide iron complex polysaccharide structure investigated for the first time, to compare the Angelica polysaccharide structure before and after synthesis Angelica polysaccharide iron complex, research Angelica polysaccharide iron complex provide a theoretical basis for the mechanism of action of the substance on the molecular level. The first part of Angelica polysaccharide ASP1 ASP2 extraction and separation, purification and structure identification by water to mention alcohol precipitation Angelica crude polysaccharide certain purity of Angelica polysaccharide the tune via alkali Shen acid after removal of protein, glucan by Sephadex G-50 gel column eluting different molecular weights of Angelica the polysaccharide ASP1 and ASP2. ASP1 a molecular weight was measured using high performance gel chromatography, the results show a single ASP1 molecular weight, weight average molecular weight of about 7.8 × 104Da; analyzed by gas chromatography after derivatization ASP1 hydrolysis results show that ASP1 the monosaccharide composition and the ratio of arabinose: glucose : galactose = 1:5.68:3.91; speculated result of the reaction by the Smith degradation, the connection mode of ASP1 1 → 4 connections and 1 → 6 connection; results can be inferred by the methylation reaction, the main chain of ASP1 mainly by 1 → 4 linked glucose and 1 → 6 linked galactose composition, the molar ratio of about 2:1; infrared spectroscopy confirmed, ASP1 mainly containing α pyranose ring structure of the β-glycosidic bond, wherein the free mannose; The result of NMR spectrum showed that ASP1 contains the following structure:-α-Ara-(1 → 4)-of β-Gala-/-β-Gala is-(1 → 4)-alpha-Glu-/-alpha-Glu-(1 → 6)-β-Gala-sephadex column ASP2 a molecular weight was measured, the results show ASP2 single molecular weight and weight average molecular weight of approximately 1620Da; ASP2 hydrolysis after derivatisation gas chromatographic analysis, the results showed that ASP2 single sugar composition as glucose; result of the reaction by the Smith degradation speculated ASP2 connection mode 1 → 4 connections and 1 → 6 is connected; ASP2 is mainly 1 → 6 connected by the results of the methylation reaction can be inferred, in part from 1 → 4 is connected a straight-chain glucan; infrared spectra confirmed, ASP2 mainly containing α-glycosidic bond of pyranose ring structure, wherein excluding mannose; result of NMR spectrum showed that ASP2 contain the following structure:-α-Glu - (1 → 6)-α-Glu-second part of Angelica polysaccharide iron complex outer sheath-like the polysaccharide ASP3 the separation and purification and structure identification of Angelica polysaccharide iron complex is FeOOH polymeric iron core for the structure of the center, in the polymerization of iron stable chelate nuclear layer of polysaccharide chain formation of iron-core molecules outside the iron core molecule and then enveloping layer of hydrophilic sheath-like polysaccharide chain. Angelica polysaccharide iron complex by Sephadex G-50 Sephadex column eluted sheath-like polysaccharide ASP3 of of the iron core and the outer layer. Dextran column method the ASP3 a molecular weight was measured, the results show a single ASP3 molecular weight, weight average molecular weight of approximately 1620Da; analyzed by gas chromatography after derivatization ASP3 hydrolysis results that the ASP3 the monosaccharide composition is glucose; by Smith degradation reaction results suggest the ASP3 the connections are 1 → 4 connections and 1 → 6 is connected; can be inferred by the results of the methylation reaction, ASP3 based connection based on 1 → 4, Part 1 → 6 straight chain connected Portugal glycan; infrared spectroscopy confirmed, ASP3 mainly as a pyranose ring structure containing α-glycosidic bond; NMR spectra ASP3 least three different chemical environment of the sugar residue. The third part of Angelica polysaccharide ASP1 the the ASP2 with angelica polysaccharide iron complexes the outer sheath polysaccharide ASP3 structure comparison ASP1 weight average molecular weight of about 7.8 × 104Da; ASP2 and ASP3 the weight average molecular weight is 1620 Da. Monosaccharide composition ASP1 and the ratio of arabinose: glucose: galactose = 1:5.68:3.91; the monosaccharide ASP2 and ASP3 are glucose. ASP1 a main chain of glucose connected by 1 → 4 and 1 → 6 linked galactose, the molar ratio of about 2:1; the ASP2 by 1 → 6 linked glucose and a small amount of 1 → 4-linked glucose; ASP3 1 → 4-linked glucose and a small amount of 1 → 6 linked glucose. ASP1 mainly comprising an α, β-glycosidic bond pyranose ring structure; ASP2 ASP3 mainly pyranose ring structure containing the α-glucoside bonds, which are all free mannose. The synthesis of Angelica polysaccharide iron complex, Angelica the polysaccharide contains ASP1 and ASP2; synthesis Angelica polysaccharide iron complex, the iron core and the outer layer of the sheath-like polysaccharide ASP3 eluted by Sephadex column. In synthetic of Angelica polysaccharide iron complex process, ASP1 polymeric iron nuclear occurrence chelate to form a stable iron core molecule, ASP2 surrounding the outside of the iron core molecules to form a sheath-like layer structure. Elution of the the angelica polysaccharide iron complex after sephadex column, the outer sheath of the iron core polysaccharide ASP3 is eluted, in the structure to theoretically ASP2 with ASP3.

Related Dissertations

  1. Study on Protective Effects of Apple Polyphenol on Immune System Injury Caused by γ-Ray,S661.1
  2. Study on Wastewater Purification Effect of the Free Surface Wetland with Different Substrates and Plant Combination,X703
  3. Penicillium Expansum TS414 Lipase in Pichia Pastori: Expression, Purification and Enantioselective Esterification of (R, S)-naproxen,Q814
  4. Studies on Fermentation Optimization, Purification and Enzyme Characteristics of Lipase from Aspergillus Oryzae FS-1,TQ925.6
  5. Studies on Isolation, Purification and Chemical Structure for Polysaccharides from Camellia Chrysantha(Hu) Tuyama,S567.19
  6. Research of Glycosaminoglycan from Perna Virids Effect on Blood Lipid,R285.5
  7. Purification of Endogenous Proteases from (Litopenaeus Vannamei) Shrimp Head and Their Enzymology Properties,S985.21
  8. Study on the Preparation of the Low Molecular Weight Fuciodan from Sargassum Henslowianum (C.Agardh) and Anti-tumor Activity,TS254.9
  9. Under microaerobic conditions closed calcium carbide furnace exhaust COS gas adsorption purification,X781
  10. Studies on Extraction and Analysis of Fructooligosaccharides in Smallanthus Sonchifolius,TS255.1
  11. Experiment Study of the Biogas Purification Process by Zeolite Filling Washing,TQ221.11
  12. Optimization of Fermentation Conditions, Purification, Cloning and Expression of a Cold-active Lipase from Pseudomonas Sp.RT-1,TQ925
  13. Extraction, Purification and Antifungal Activity of Anthocyanins in Drop Fruits of Chinese Baberry,TQ914.1
  14. Preliminary Research on Characterization of Thermophilic Protease Pasey and Helicase Htc16,Q814
  15. Study on the Separation and Purification Way of Arctiin from Fructus Arctii and the Effect of Arctiin on the Growth of Mice C2C12 Cells,S853.7
  16. Purification of Human Respiratory Syncytial Virus Fusion Protein with Immunomagnetic Microsphere Technology,R373
  17. Identification of Marine Sreptomyces Sp. GY-4 and Researches on Its Antimicrobial Substances,Q936
  18. Prokaryotic Expression and Purification of Human Beta-Defensin-9,Q78
  19. The Research of Synthesis of Bisphenol-A Polycarbonate Through Melt Transesterification Process,TQ323.41
  20. Screening of Bacterial Strains with High α-Galactosidase Activity and Its Gene Cloning, Expression, Purification and Characterization,TQ925
  21. Study on Extraction, Isolation and Antioxidant Activity of Lignans of Sesame Cake,TS229

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Traditional Chinese medicine chemical > Chemical analysis and identification
© 2012 www.DissertationTopic.Net  Mobile