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Objective To study the grape seed proanthocyanidin extract (GSPE) human Tenon capsule fibroblasts (Tenon's capsule fibroblasts, TFb) proliferation, migration impact . Treated separately with different concentrations of GSPE Tenon's capsule fibroblasts 24h , cell scratch assay TFb of transitional cell viability by MTT assay , immunohistochemistry assay TFb p21 protein expression . The results compared with the control group , when the concentration of gt; 10ug/ml , GSPE significantly inhibit TFb proliferation , the difference was statistically significant ( P lt; 0.01 ) . Inhibition between the different concentration with increasing drug concentration , inhibition enhanced between each concentration group difference was statistically significant ( P lt; 0.01 ) . 2 cells scratch method shows the of GSPE processing cells of different concentrations for 24 h after the number of cell migration for the negative control group 46.80 ± 11.35,29.60 ± 7.20,10.80 ± 5.50 86.00 ± 11.47 treated group and control group differences compared statistically significant (P lt; 0.01), the difference between groups was also significant ( P lt; 0.01 ) . 3 immunocytochemistry : increased expression of p21 protein GSPE, it was in each dose group , compared with the negative control group , the difference was statistically significant ( P lt; 0.01 ) . Conclusion GSPE inhibit TFb transitional , and by upregulating the expression of p21 protein inhibits cell proliferation , GSPE, it was possible role in the formation of anti-glaucoma filtration surgery bleb scarring worthy of further study .
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