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OBJECTIVE: To compare high-frequency (frequency 100HZ, voltage 3V, continuous wave), intermediate frequency (frequency 50HZ, voltage 3V, continuous wave), low frequency (frequency 2HZ, voltage 3V continuous wave) electroacupuncture preconditioning induced acute coronary artery ligation myocardial ischemia model in rabbits heart damage protective effect and the effect of its protection mechanisms, to provide experimental evidence for the clinical application of universal application acupuncture treatment of ischemic heart disease. Methods: According to Xu Shuyun recommended method of rabbits with acute myocardial ischemia model copied by coronary artery ligation, BL-420F biological and functional experimental system records II-lead ECG and left ventricular pressure, determination of serum LDH 722 spectrophotometer CK, CK-MB, SOD, MDA content and NBT staining method estimates myocardial infarct size were observed. Select 1.5 ~ 2.5kg healthy adult rabbits, BL-420F biological and functional experimental system recorded its II-lead ECG, and ECG abnormalities were excluded, the ECG normal (male and female) were randomly divided into normal group, model group, sham group, the control group, high, medium, low-frequency electro-acupuncture group, 10 in each group. High, medium, low-frequency electro-acupuncture group were given seven days electroacupuncture pretreatment acupoint selection bilateral Neiguan EA once daily from 8:00 to 10:00, each EA 40min Hua Tuo Brand 0.30 × 40mm needles, rabbits off acupoints reference spectra of of Experimental Acupuncture Animal standard acupoint, independent of electrode in the palm connected G6805-Ⅱ electro-acupuncture device, electro-acupuncture parameters: the low-frequency group 2Hz, the IF group 50Hz, high group 100Hz, the voltage is 3V, continuous wave. Normal group, sham operation group, model group, the control group does not give EA stimulation, but be bundled the similar acupuncture group equal time seven days, so that all animals are compared under the same conditions. Each group final processing is completed immediately with 20% urethane 5ml/kg ear vein injection anesthesia, supine position fixed on the stage, cut the neck, chest hair, iodine disinfection, alcohol deiodination, shop towel hole after sterile surgical separation the trachea pick animal ventilator, the separation of the right common carotid artery and insert a cardiac catheter recording of left ventricular pressure curve, and inserted in the limbs subcutaneous needle electrode recording II lead ECG curve. Normal group not exposed heart; surgical exposure to the heart of the sham-operated group, and then separation of the left anterior descending artery ligation; model group, EA group were exposed to the heart, separation coronary artery and threading ligation of the left anterior descending artery; surgical exposure of the control group heart, separation of the left anterior descending coronary artery, the the threading first after blocking blood flow 5min, reperfusion 5min so repeated 3 times for 30min, then ligation of the left anterior descending artery. Each group record after coronary artery occlusion 10s, 1min, 5min, 10min, 30min, 60min, 90min, 120min, 150min, 180min time point II-lead ECG and left ventricular pressure changes; blood in the left common carotid artery ligation 360min 4ml, 4 ℃ centrifugal separation of the serum, the press kit require determination of the serum of LDH, CK, CK-MB, SOD, the content of MDA; carotid blood, the heart was removed immediately cut open the chest cavity, along with ice brine after Coronal removed great vessels and atrium, left ventricle uniform cross-section 5, by NBT staining estimated range of myocardial infarction. Results: model group, heart rate slowed down, the T-wave high tip, ST segment changes significantly, compared with normal group by the presence of statistically significant difference; examination revealed left ventricular pressure, reduced model group LVSP, LVEDP increased, dp / dtmax significant difference, -dp/dtmax slowed down compared with normal group; model serum LDH, CK, CK-MB rise increased, MDA, SOD decreased, compared with the normal group exist significant differences; model of myocardial infarct size there is a significant difference compared with the normal group. High, intermediate frequency electric needle group T-wave magnitude of the changes and ST segment significantly improved, significantly improved low-frequency electric needle group heart rate, with the model group comparison exists significant difference; significantly improve the role of high, intermediate frequency electric needle group LVSP, dp / dtmax significant differences exist; IF EA group the LVEDP -dp/dtmax improved significantly with the model group differences exist; high, medium and low-frequency electro-acupuncture serum LDH, CK, CK-MB decreased compared with the model group, with the model group were significant differences; high increased intermediate frequency electric needle group serum SOD, MDA reduce, with the model group, comparing differences in the presence significantly with sex; low frequency electric needle group serum of SOD, of MDA changes with the model group comparison no significant difference; myocardial infarction range of high-, intermediate-frequency electro-acupuncture group reduced significantly with the model group differences exist, no significant changes in myocardial infarction range of low-frequency electro-acupuncture group compared with model group differences. Indicators in the control group compared with model group differences were significant. Conclusion: 1, ligation of the left coronary artery of rabbits before descending branch of the successful replication of acute myocardial ischemia animal model, significant changes in the observed indicators. 2 different frequency acupuncture pretreatment of coronary artery ligation rabbit heart damage caused by acute myocardial ischemia model has a protective effect. 3, acupuncture protective effect of SOD activity and MDA. 4 acupuncture protective effect of improvement in left ventricular systolic pressure, left ventricular terminally diastolic blood pressure and left ventricular pressure related to the maximum rate of change. 5, acupuncture can be used as a means of anti-ischemic pretreatment, but still need to conduct in-depth research.
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