Dissertation > Excellent graduate degree dissertation topics show

Survival and Differentiation of Autologous Bone Marrow Mesenchymal Stem Cells in Injured Spinal Cord

Author: ShenZhongJie
Tutor: LiaoWenBo
School: Zunyi Medical College,
Course: Orthopedic Surgery
Keywords: autologous bone marrow mesenchymal stem cells spinal cord injury transplantation survival differentiation
CLC: R651.2
Type: Master's thesis
Year: 2011
Downloads: 19
Quote: 0
Read: Download Dissertation

Abstract


Objective:To observe survival and differentiation of autologous bone marrow mesenchymal stem cells(BMSCs) in semitransected spinal cord.Methods:36 male SD rats were randomly divided into BMSCs autograft group (ie, experimental group) and PBS control group, for each group of 18 rats.The rats of autologous BMSCs transplantation were intercepted on the left tibia and fibula in order to collect bone marrow, and then use the method of density gradient centrifugation combined adherent to purificate and culture BMSCs.The phenotype of the fourth generation of BMSCs was identified by flow cytometry.3ul (cell density of 5 x 103/ul) of autologous the fourth generation of singel cell suspension BMSCs labeled with Hoechst33342 were immediately transplanted respectively into the semitransected site and at its upper and lower the range of 3mm. PBS control group was respectively injected 3ul PBS solution in the same site.Two weeks、three weeks and five weeks after post-transplantation, the rats of autologous BMSCs transplantation were killed to observe the survival and detect the expression of neurocyte specific proteins of MAP-2, GFAP by using immunofluorescence staining.Results:①In the fourth generation of BMSCs, the expression level of CD29 and CD90 were 97.17% and 99.49% respectively, but the hematopoietic stem cells surface maker such asCD45 was absent.②Two weeks、three weeks and five weeks after transplantation,the BMSCs labeled with Hoechst 33342 were observed around the injection spot more cells survive at two weeks after transplantation and less cells at five weeks.③Two weeks and three weeks after transplantation,those surviving BMSCs did not express MAP-2 and GFAP, but the MAP-2 and the GFAP could be dectetived in some of surviving BMSCs at five weeks after transplantation.Conclusion:①By using the method of density gradient centrifugation combined adherent to purificate and culture BMSCs,the fourth generation of BMSCs has high purity, and those cells can be used for subsequent cell transplantation experiments.②At the moment of spinal cord injury, autologous BMSCs can survive in the injured spinal cord and a few of them can differentiate into neuron-like cells and astrocyte-like cells.

Related Dissertations

  1. Study of Intracerebral Transplantation of Umbilical Cord Blood Mesenchymal Stem Cells in a 6-Hydropamine Rat Model of Pakinson’s Disease,R742.5
  2. The Research of Radiobiological Effect Mathematic Model Based on Equivalent Uniform Dose,R311
  3. Fine Root Longevities of Two Subtropical Evergreen Broad-leaved Forests,S718.5
  4. Effects of Temperature, Salinity, Dissolved Oxygen, Ammonia-N and Nitrite-Non the Molting of Scylla Paramamosain,S917.4
  5. Study on Characteristics of Eremochloa Ophiuroides Germplasm in the Hilly Areas of Ningzhen,S688.4
  6. A Model of Acute Interstitial Pneumonitis by Murine Cytomegalovirus Infection in Mice with Allogenic Skin Transplantation,R-332
  7. The Construction and Evaluation of a Novel Non-viral Gene Transfection System and Its Application in Mesenchymal Stem Cells Gene Recombination,R346
  8. Effects of Optimized Main Environmental Factors at Broilers Raising and Scutellaria Baicalensis on Production Performance of Three Yellow Broilees,S831.5
  9. The Study of Immuno-tolerance Mechanism of the Third-party Bone Marrow-derived Mesenchymal Stem Cells on Allogenic Transplantation,R392
  10. Clinical Research of Autologous Bone Marrow Stem Cell Transplantation on Liver Cirrhosis Induced by Hepatitis B,R575.2
  11. Anesthesia Management of Cervical Spinal Cord Injury,R614
  12. Old Ⅲ Colorectal Cancer Survival after Radical Analysis and Evaluation of Chemotherapy,R735.3
  13. Clinical Research of Autologous Bone Marrow Stem Cell Transplantation for Treatment of Decompensated in Cirrhosis,R575.2
  14. Experimental Investigation of Grafting Pathways of Bone Marrow Stromal Cells into Spinal Cord Injury in Rats.,R651.2
  15. 3 T Magnetic Resonance Diffusion Tensor Imaging and Fiber Tracking in Detecting Acute Spinal Cord Injury,R651.2
  16. The Meaning of Blood Lactic Acid Dynamic Monitoring and Continuous Hemodynamic Monitoring of Severe Case Trauma Patients,R641
  17. Improvement of the Result of Autologous Fat Transplantation in Facial Recontouring Surgery by Using "Cocktail" Therapy,R622
  18. Clinical Effect of Autologous Fat Pellet Particle Transplanting Combined Ligustrazine on Breast Augmentation,R622
  19. The Study of the Influence of BMS-345541 on Motor Function of Hind Limbs and the Infiltrating Leukocytes after Spinal Cord Injury in Rats,R651.2
  20. Applied Clinical Research toward Improving Fat Cell Survival Rate in Autologous Fat Transplantation Breast Augmentation,R622
  21. Association of Expression of ERCC1/RRM1 with Survival and Resistance to Platinum Inpatients with Ⅰ-ⅢA NSCLC,R734.2

CLC: > Medicine, health > Surgery > Of surgery > Head and Neurosurgery > Spinal cord
© 2012 www.DissertationTopic.Net  Mobile