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The Sox genes Dmrt gene family two gene families involved in the biological ontogeny, research at the molecular level, to understand its members molecular evolution and its specific take on important biological functions. The Sox genes contain a highly conserved DNA specific binding motif HMG-box, regulating animal sex determination and differentiation process, and participate in a variety of organ development. Dmrt gene family is a class of newly discovered containing DM conservative box transcription factor, combined zinc finger structure with specific DNA sequences involved in regulating animal sex determination and differentiation, organ development and function to maintain. In this paper, part of an overview of the four aspects of the Sox gene family characteristics, classification, function and molecular evolution, an overview of the research through the features and functions of the Dmrt genes. Report in this paper, with reference to the human SRY gene HMG-box conserved sequence, designed a pair of degenerate primers, the nigromaculata Sox gene were amplified by PCR, and a size of approximately 220bp were amplified in male and female nigromaculata individuals a strip, the amplification product was cloned, the positive clones using the SSCP analysis, screening fragments of the different genes. DNA sequencing further screening of different genes, the results of four different Sox genes with GenBank online, using the BLAST sequence alignment of human SOX gene and found that the DNA sequence identity with the corresponding human SOX gene respectively was 86%, 83%, 82%, 89%, 94% amino acid sequence identity with the corresponding SOX protein, respectively, 93%, 78%, 98%, showing that the gene is highly conserved molecular evolution. Based on the spots frog (Rana nigromaculata), the Latin name, the customary name rn Sox3 the rn Sox21 rn Sox4, rnSox11. The article refers to a different species DM domain design another pair of degenerate primers, dark spots the frog Dmrt gene was amplified by PCR technology, results in a male and female individuals were amplified with a length of about 140 bp. The amplified product was cloned, and SSCP analysis of positive clones were used to filter the different gene fragments and DNA sequencing, further screening of different genes. Obtained a three different Dmrt gene results online in GenBank using BLAST method with the corresponding human DMRT gene sequence alignment, the amino acid sequence identity with the corresponding human DMRT gene were: 89%, 93%, 89%, significantly
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