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Rabies is caused by the rabies virus zoonotic deadly infectious diseases , is so far the highest fatality rate of human acute infectious diseases , once the disease of humans and animals , the mortality rate as high as 100 % , yet he is still widespread concern . The monoclonal antibody anti-rabies virus ( RV ) is the study of rabies virus diagnosis , treatment and prevention of rabies powerful tool . In order to obtain high titer , high specificity anti- RV monoclonal antibody, and used in fluorescence detection . The experimental RV SRV9 strains inoculated suckling mice , collected after the onset of cerebral charged by the homogenate centrifuged supernatant , RT - PCR analysis TCID50 determination to prove that the product contains high titers of rabies virus . The virus immune Balb / c mice spleen cells and SP2 / 0 myeloma cells by indirect ELISA screening , 15 stable hybridoma cell lines secreting anti- RV antibody cloned by limiting dilution , and ultimately , were named C1, C2, C3, C4 ...... C15. Ascites production of monoclonal antibody of the hybridoma strain inoculation of 15 mAbs Balb / c mice by intraperitoneal , ELISA determination of the titer up to l : 2 x 103 - l.6 × 106 . Indirect ELISA and Western-blotting test results show that the 15 monoclonal antibody with the RV SRV9 whole virus antigen with different families virus canine distemper virus ( CDV ) , canine parvovirus ( CPV ) , canine adenovirus ( CAV ) and blisters stomatitis virus (VSV) are not cross-react , and of these 15 strains with good specificity monoclonal antibody RV ; wherein C1 , C4, C8, C9, C10, C13 , C15 mAb with SRV9 , 8202 , CVS the three strains reactions show that they are common antigen epitope monoclonal antibody against the rabies virus . MAb C1 , C2, C5 , C8 , C9 , C15 belongs to the IgG2a subclass antibodies , mAb C4 , C10 , C13 belongs to the IgG1 subclass antibodies , the remaining six strains of monoclonal antibody are IgG2b subclass antibody . Labeled with FITC ( fluorescein isothiocyanate ) , the purified monoclonal antibody ascites by fluorescent focus inhibition test RV antibodies in the serum of dogs to detect and determine the titer . Been known positive serum of known RV RV negative serum and tested serum sample test results show that the method is sensitive, specific and rapid .
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