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Screen and Analysis of Specific Expression Gene Promoter in Stem and Leaf of Rice

Author: LaiPengCheng
Tutor: WangFeng
School: Fujian Normal University
Course: Biochemistry and Molecular Biology
Keywords: Oryza sativa L promoter stem and leaf-specific promoter
CLC: S511
Type: Master's thesis
Year: 2011
Downloads: 16
Quote: 0
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Abstract


This study of rice stem leaf in the early advantage OsZF153 expressed genes OsW8513 promoters and missing function on the basis of analysis, further explore the two promoter function. One is missing a series of OsZF153 to early promoter transgenic rice plant validation, and build a 3’end 1407bp promoter for missing the length of P153E and pCxgus expression vector into 5’-the 100bp promoter forward pCxgus-P153m expression vector.2 it is the lack of a series of early OsW8513 promoter, and constructs the transgenic plants validation pCxgus-two expression vector P8513G and pCxgus-8513E. These four carrier through agrobacterium-mediated transformation Japan fine rice embryo callus. Mature of maize and tillering stage, genetically modified rice plant roots, stems, leaves, flowers, clever shell and seed each organ GUS activity detection.Through bioinformatic approaches, screening may be stem leaf specific promoter, then use RT-PCR methods validation gene expression patterns in the database. To the candidate sl5 and sl2 gene promoter and gene promoter bioinformatics analysis, forecast the cis-element, speculated that stem leaf specific gene promoter may have the cis-regulatory function components.

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CLC: > Agricultural Sciences > Crop > Cereal crops > Rice
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