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The rubber tree is extremely important cash crops in China 's tropical and subtropical regions . Narrow rubber tree germplasm sources of material , after years of selective breeding and related hybrid , small external morphological differences between the varieties of materials used in the production and breeding , some full-sib and half-sib progeny morphological methods , cytology , even protein labeling method are difficult to distinguish . DNA molecular markers appear International Login rubber tree varieties and new varieties of protection from the molecular level to provide a reliable species identification techniques . Therefore, in order to identify the varieties of rubber trees to study the phylogenetic relationships among species , in order to better protect the use of this excellent germplasm resources , the use of RAPD markers , a preliminary analysis of 39 rubber tree fine varieties of DNA fingerprinting to obtain the following main results : 1 700 10 base random primers screening last 17 ??clear amplified bands , polymorphic primers . And then these 17 primers for amplification of 39 rubber tree varieties , the amplified bands of 100 different primers amplified DNA bands in the range of 4 to 10 , with 5.88 per primer . Polymorphic bands 78 , 78% polymorphism ; 2 RAPD fingerprint application of primer F15 built , one-time to the identification of nine species open to other primers constructed map of the test material can be Qi classification to determine each RAPD profiles in different bands , according to each specific band or different bands can also distinguish between the 22 samples tested , the remaining eight species can not be identified ; tested 39 varieties Simple Matching similar factor analysis and cluster analysis , the similarity coefficients range between 0.64 to 0.95 , consistent clustering results and pedigree .
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