Dissertation > Excellent graduate degree dissertation topics show

Apoptosis of K562 Cells Induced by Glucosamine Sulfate and Mechanism of the Signal Transmit

Author: PuZuo
Tutor: LiuLiMei；WangZhe
School: Fourth Military Medical University
Course: Internal Medicine
Keywords: glucosamine sulfate (GS) K562 apoptosis cathepsinD Bcl-2 family
CLC: R733.7
Type: Master's thesis
Year: 2005
Downloads: 67
Quote: 0
Read: Download Dissertation

Abstract


Abstract: AIM To induce of PCD of leukemia cells is an important strategy in leukemia therapy.To find inducers of apoptosis which have lower side effect and obvious curative effect have been put to the front in new tumor therapeutic drug investigation and development. Better knowledge of cellular mechanisms that regulate apoptosis will be accompanied by further understanding of mechanism of apoptosis. We investigate the effect of glucosamine sulfate(GS) on chronic myloid leukemia K562 cells and the possible functions of Bcl-2 family proteins in the cathepsin D mediated apoptosis of K562 cells induced by GS. METHODS Electron photomicroscopy and Wright ’s-Giemsa staining were used to study the morphologic evidences for apoptosis of K562 cells induced by glucosamine sulfate (GS). Cell cycle and Annexin V were detected by flow cytometry. The analysis of DNA Ladder was tested by agarose gel electrophoresis. Immunofluorescence was used to observe the translocation of cathepsinD and cytochromeC during the apoptosis. The expression of caspase-3、 cathepsin D、 bcl-2 bcl-xL、 bid and bax in K562 cells treated by GS were detected by using western blot. RESULTS Treatment with 0.5,1,5mmol/L GS resulted in a concentration-dependent and time-dependent reduction in K562 cells

Related Dissertations

  1. The Study on Cryopreservation and Mechanism of Freezing Injury on the Spermatozoa of Coelomactra Antiquate,S968.3
  2. The Anti-tumor Effect of CADPE and Inducing Apoptosis in Human Gastric Cancer Cells,R735.2
  3. Cloth and β- elemene combined administration of anti-tumor effect and mechanism of celecoxib,R96
  4. TRAIL in the regulation of tumor invasion CD4 ~ CD25 ~ Treg,R730.2
  5. The Study of Lead Exposure on Cyprinus Carpio Ovary Epithelial Cells,X174
  6. Effects of Diclazuril on G3PDH in Second-generation Merozoites of Eimeria Tenella,S858.31
  7. The Effects and Preliminary Study of PCV2 on Ca2+ Signal in Lymphocytes of Piglets,S858.28
  8. Effects of Angiogenic Factor on Follicular Angiogenesis and Development in Sexual Maturity Mice,S852.2
  9. Expression of β-Catenin in Pig’s Ovary and the Effect of β-Catenin on Porcine Granulosa Cells Apotosis and Steroidogenesis Related Enzyme,S828
  10. Induced Combination Apoptosis by FB1 and AFB1 in Vero Cell,S856.9
  11. The Apoptosis Mechanism Induced by Aflatoxin B1 and Deoxynivalenol in Primary Hepatocyte of Cyprinus Carpio,S856.9
  12. Effects of BMPR-IB Gene Silencing by Small Interfering RNA on Apoptosis of Porcine Pollicular Granulosa Cells and Ecpression of BMP Pathy-Way-Ralted Genes,S828
  13. The Effects of Don on Proliferation, Differentiation, and Apoptosis of Chondrocytes in Chicken,S858.31
  14. The Effect of Foxol on Apoptosis of Mouse Granule Cells,S865.13
  15. Study of Melittin on Tumor Inhibition and Part of Mechanism of Human Hepatocellular Carcinoma HepG-2 Xenograft in Nude Mice,R735.7
  16. The Protective Effect of Rat Myocardial Ischemia/reperfusion Injury Following Bone Marrow Mesenchymal Stem Cell Pretransplantation for 1 Week,R542.22
  17. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  18. The Effects of (180F-FDG on the Apoptosis of Eca-109 Esophageal Cancer Cell Line,R735.1
  19. The Signal Mechanism Underlying DON Induced Apoptosis in BHK-21 Cells,S856.9
  20. The Expression and Mechanism of NF-κ B and IL-6 after 90% Portal Vein Ligation in Rats,R657.3
  21. The Study of Mitochondrial Signal Mechanism Induced Apoptosis by Don in Human Colon Cancer Cells,R735.35

CLC: > Medicine, health > Oncology > Hematopoietic and lymphoid neoplasms > Leukemia
© 2012 www.DissertationTopic.Net  Mobile