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Objective : Diabetic nephropathy (diabetic nephropathy, DN) is the most harmful microvascular complications of diabetes , is one of death, leading causes of disability . Epidemiological studies show that in the United States , end-stage renal disease (ESRD) about 1/ 3 of diabetes-induced and 75% of type 2 diabetes , our approximately 5%. After the illness worse , death rate , seriously affecting people's quality of life. At home and abroad there is no ideal drug for this disease . Typical pathological changes of diabetic nephropathy , including renal hypertrophy , basement membrane thickening , glomerular accumulation of extracellular matrix components and so on. In recent years with advances in molecular biology techniques , found extracellular and intracellular factors on cell proliferation and growth have occurred in the final level of the cell cycle . With cells (apoptosis, APO) deepening of the study prove that apoptotic mechanisms involved in the occurrence of DN and is causing further deterioration of renal function is one important factor . Study found that kidney cells in the presence Fas / Fas-L system , and participated in the diabetic rat kidney cell apoptosis . Our hospital laboratory using kidney qi righting Huoxuetongluo prognosis for kidney detoxification Jiangzhuo mixture ( rhubarb , astragalus , mistletoe , Chuanxiong, leeches, Codonopsis, blood over charcoal , Poria , etc. ) treatment of the disease , and achieved good effect. We copied diabetic nephropathy rats , by observing the more kidney mixture on renal function , cell cycle, apoptosis and related proteins Fas / Fas-L expression , and to explore the side of the therapeutic mechanism for the clinical treatment of DN provide experimental basis . Methods: 60 healthy SD rats were randomly selected 10 of the sham group
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