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Purpose: corneal alkali burns led to the formation of new blood vessels is difficult to restore their eyesight is a major problem . Recent studies suggest that vascular endothelial growth factor (VEGF), matrix metalloproteinase -9 (MMP-9) and nitric oxide (NO) and in vivo angiogenesis are closely related. This study was prepared by alkaline burns rabbit model of corneal neovascularization , corneal alkali burn corneal neovascularization in different periods of growth , positioning and semi-quantitative analysis of corneal tissue VEGF and MMP-9 expression , detection of corneal NO content , and with a nitric oxide synthase inhibitor N- nitro -L-arginine methyl ester (L-NAME) group compared to the same time observed after alkali burn corneal epithelial tissue ultrastructure, to investigate VEGF, MMP- 9 and NO on corneal neovascularization , understanding L-NAME in promoting healing of corneal epithelial damage in the role with a view of corneal neovascularization after alkali burn prevention and the promotion of corneal epithelial healing play a guiding role . Methods: 40 healthy New Zealand white rabbits were prepared corneal neovascularization , left for the experimental eye . Randomly divided into 40 experimental eyes alkali burn group ( alkali burns ) and L-NAME group ( alkali burns L-NAME), n = 20 . L-NAME group 10mg/kg/d dose by intraperitoneal injection . Slit lamp corneal neovascularization growth . Two groups of animals were 1,4,7,14 days after burn each randomly selected five air embolism death , removal of the left eye, take the cornea , where 1 /2 of the corneal tissue with a nitrate reductase assay NO content ; 1/4 of the cornea and fixed with 4% paraformaldehyde solution, embedded in paraffin, made ??of tissue sections , HE and immunohistochemical staining (SP method ) , light microscopy of VEGF and MMP-9 expression in the cornea and using a computer - image analyzer its surface density
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