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Objective: Chalei Pa neomycin eye drops inhibited corneal alkali burn induced neovascularization experimental results of corneal neovascularization and inflammatory cell infiltration, vascular endothelial growth factor expression, CD34 expression of the relationship between the initial order investigate the inhibition of rapamycin eye drops corneal neovascularization in ocular mechanisms and application security. Methods: 48 healthy adult New Zealand white rabbits, eliminate eye diseases, eye neovascularization model made of alkali burns. Were randomly divided into four groups, A group (0.1% rapamycin eye drops), B group (0.5% rapamycin eye drops), C group (1% rapamycin eye drops), D group (excipient control group), eye four times daily. After Levofloxacin trachoma liquid drops 3 times a day to prevent infection. The day of surgery and the next day observation corneal opacity, dissolution and growth of new blood vessels and measure the length. Different time points after alkali burn (7,14 days) animals were sacrificed remove the cornea, HE staining of corneal tissue pathological changes of corneal infiltration of inflammatory cells were counted, OK CD34, VEGF immunohistochemical detection, counting CD34 marker neovascularization density applications pathological image analyzer VEGF expression gray value. Results: alkali burn corneal opacity in each group were significantly, the first 7 days no significant difference (P gt; 0.05); with time controls corneal opacity, dissolve heavier, the first 14 days A, D each group one case of corneal perforation , B, C group than before to reduce corneal opacity, ulcers partially restored, hypopyon has been absorbed, the first 14 days treatment group B, C and control groups corneal opacity and corneal melting difference was statistically significant (P lt; 0.05 ). Slit lamp observation 24 hours See limbal vascular network filling, the first 3d visible limbal corneal neovascularization to grow, the first 7 d to 11d corneal neovascularization vigorous growth area increases after the first 11d B, C group CNV area growth trend in stability. Neovascularization treated area at each time point were lower than the control group and the treatment group, the smaller the higher the concentration of neovascular area, where at each time point B, C group and the control group was significantly (P lt; 0.05) , A group and the control group showed no significant difference (P gt; 0.05); B, C group at 7 days, 9 days, 11 days, 14 days of corneal neovascularization area of ??difference was statistically significant (P lt; 0.05 ). B, C group inflammatory cell count, microvessel density and VEGF expression were lower than the control group, the difference was statistically significant (P lt; 0.05). CNV area and keratitis each group of cells and VEGF expression was positively correlated (P lt; 0.05). Conclusion: with a certain concentration of rapamycin inhibited corneal alkali burn inflammation and neovascularization role for corneal neovascularization after alkali burn treatment provides a safe and effective method. Corneal alkali burns inflammatory cell infiltration and increased expression of VEGF, suggesting that inflammatory cells and VEGF and CNV is closely related, both rapamycin may inhibit angiogenesis through endothelial cells, inflammation, cell proliferation, inhibition of VEGF expression in a variety of means to suppress the occurrence of CNV.
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