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Objective: To study of bevacizumab in the treatment of corneal neovascularization in rabbits and drug concentration and the role of the correlation . Method: 1, 48 healthy rabbits were randomly divided into saline control group and different concentrations of bevacizumab treatment group ( 1.25mg/ml , 3.75mg/ml , 6.25 mg , 12.5mg/mL 25.0 mg / ml ) . 2 , alkali burns to establish the model of corneal neovascularization , corneal neovascularization after alkali burn pathological changes observed under light microscope ; qualitative observations of vascular endothelial growth factor , matrix metalloproteinase- 2 expression by immunohistochemistry ; RT - PCR detection of VEGF mRNA expression . Results: 1 , morphological observation: to slow the growth of neovascular buds of the treatment group compared with the control group , small diameter , 6.25 mg and above concentrations of Avastin group more significant . 2, the length and area of corneal neovascularization statistical results : Compared with the control group , 6.25 mg and above concentration Avastin group neovascularization length and area were significantly reduced, the differences were statistically significant ( P lt ; 0.01 ) . 6.25mg/ml, 12.5mg/ml, 25.0mg/ml Avastin group compared with no significant difference ( P gt ; 0.05 ) 3 , VEGF , MMP - 2 immune histochemical determination results : the treatment group, the expression of inflammatory cells was ( ) , a significant difference compared with the control group . Gray value results : 1.25mg/ml , 3.75mg/ml Avastin group compared with the control group , the difference was not statistically significant ( t gt ; 0.05 ) ; 6.25 mg and above concentration of Avastin group and control group , 1.25mg / ml , 3.75mg/ml Avastin group compared to the differences were statistically significant ( t lt ; 0.01 ) , but the concentration of more than 6.25 mg Avastin group compared with no statistically significant difference ( t gt ; 0.05 ) Real - time PCR results : 1.25mg/ml , 3.75mg/ml Avastin group compared with the control group , VEGF mRNA gene expression level is relatively high , 6.25mg/ml, 12.5mg/ml, 25.0mg/ml Avastin group VEGF mRNA gene expression levels were significantly reduced , and no significant difference between the three groups after . Conclusion: bevacizumab on corneal neovascularization inhibition with increasing drug concentration gradually increased , when the drug reaches a certain concentration , inhibition stabilized .
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