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Of angiostatin (angiostatin), generated as a specific blood vessel inhibitor negative regulator involved in tumor angiogenesis , inhibition of tumor growth and metastasis has an important role . Therefore , the study of their generation mechanism has attracted widespread attention . The study showed that : angiostatin Plasminogen internal structure of the part , some protease , to release by hydrolysis of plasminogen containing angiostatin structure and biological activity of the fragments . In-depth study of these protease prompted angiostatin not only contribute to the further understanding of the regulatory mechanisms of angiogenesis , and may provide new clues for tumor anti-angiogenesis therapy and means . In this study, by two specific cleavage of plasminogen original identification of the protease , of bacterial origin and sources of tumor cells to explore angiostatin formation routes . Room Dr. Li Fuyang found a hydrophila Aeromonas secreted serine protease , plasminogen into angiostatin . Protein purification, mass spectrometry and amino acid sequence determination , to obtain the amino acid sequence of the the 8 peptide fragment of the protease . Based on the basis of the above work , the results of this experiment according to the obtained partial amino acid sequence, and its homology analysis in the protein database , design of the probe , the establishment hydrophila Aeromonas genomic library by the hybridization method , the library Filter to possible coding purposes protease gene fragment by genome walking technology
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