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Tissue Engineering Cartilage Generated from Cryogenic Chondrocytes and Cryogenically Preserved

Author: LiJunZheng
Tutor: ChengShiYin；CuiPengCheng
School: Fourth Military Medical University
Course: Department of Otolaryngology,
Keywords: Cryopreservation Cartilage Chondrocytes Tissue Engineering Transplant Polyglycolic acid
CLC: R318.0
Type: Master's thesis
Year: 2005
Downloads: 61
Quote: 0
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Abstract


Congenital and acquired the repair of cartilage defects has been a major problem in clinical autologous chondrocyte transplantation is the ideal method for repair of cartilage defects of the Head and Neck Surgery . However , this approach requires from the patient autologous take cartilage, not only add to the economic, physical and psychological burden of the patient , the more important is the limited number of autologous cartilage can provide . The emergence of tissue engineering technology to solve this problem and hope that application cartilage chondrocytes in vitro . Source of seed cells for cartilage tissue engineering foundation and core content , how to save a lot of cartilage cells , in a timely manner to provide adequate preservation of the seed cell and tissue-engineered cartilage tissue engineering cartilage is an unresolved issue in cartilage tissue engineering , built the experimental application of cryopreservation chondrocytes tissue engineered cartilage tissue engineering cartilage cryopreservation, the feasibility of a preliminary study . Lab purpose to study cryopreservation cartilage cells as seed cells to construct tissue engineered cartilage the ability . Methods 2 -week-old New Zealand rabbit articular chondrocytes , about Cryopreservation recovery and in vitro culture , take the 3rd generation of cultured cells in logarithmic growth phase , the cell suspension , adjust its 5 × 10 ~ 7 / ml inoculated cultured for one week in the three - dimensional scaffold of the PGA , composite objects , transplantation in the New Zealand rabbit , after 12 weeks , gross and histological observation . Result

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