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Animal studies of the safety of two rare earth nano material

Author: QinZuoXia
Tutor: MaJianWei
School: Qingdao University
Course: Textile Engineering
Keywords: Nano Antibacterial Toxicity Immunity Genetic material
CLC: R318.08
Type: Master's thesis
Year: 2005
Downloads: 177
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Abstract


Preliminary study of the subject from the point of view of immunology and health toxicology two rare earth nano material impact on guinea pig immune function and genetic material, and certain organs pathological analysis Finally, there may be the mechanism discussed to provide some reference period for the study of rare earth nano material safety. First, according to the assessment of foreign compounds under the Health Toxicology body's toxic effects, combined with rare earth nano material used in the practical application of the environment and rare earth toxicity research to determine the subject of the test design, ie : Subchronic cytotoxicity test methods, blotted out through the skin, feeding, three different injection (intravenous and intramuscular) routes of exposure, testing two different time periods of the nanomaterials on guinea pig immune level (including the humoral immune cellular immunity) and the impact of the genetic material, and certain organs, such as skin, stomach, liver, kidney, spleen, and bone marrow pathology analysis. Then, from the point of view of the humoral immune serum circulating immune complexes (CIC) determination the test (PEG test) and total serum complement (CH 5 0) determination tests, the results show: ① In addition to skin contact group, compared to the other groups guinea pig serum circulating immune complexes with control group was significantly higher; but medication for 73 days after the guinea pigs level of immune complexes with the medication 25 days of the guinea pigs did not decrease compared to the normal level, of excessive The immune complexes generated immune suppression, and after the deposition of the important factors of tissue and organ damage. ② In addition to skin contact group, other test group guinea pig serum complement the total control group was significantly different compared. The same time, they were carried out from the the cellular immunological point of view and Genetic Toxicology angle detection of T lymphocyte subsets (TLc), erythrocyte fragility detect macrophage phagocytosis test, chromosome aberration of bone marrow cells and bone marrow micronucleus analysis of five trials The results showed that: ① T lymphocyte subsets measured results show that the addition to skin contact group, CD3 cells (equivalent to the total number of T cells), CD4 of cells (equivalent auxiliary T cells) ratio and CD4 of, / CD8 of values ??were significantly reduced, while the proportion of CD8 cells (the equivalent of suppressor T cells) but increased. The above-mentioned changes in the cellular immune dysfunction, immune regulation disorder. ② erythrocyte fragility test, rare earth nano material guinea pig red cell membrane (except skin contact group) caused greater damage to the red blood cell hemolysis fragility significantly increased, indicating that the rare earth nano material may cause autoimmune hemolytic guinea pigs anemia and other diseases. (3) macrophages the phagocytosis test results show that: guinea pig exposure to rare earth nano material, in addition to skin contact group, guinea pig peritoneal macrophage phagocytosis rate compared with the control group reduce very significantly the phagocytic activity decreased, weakened immune function. ④ In addition to skin contact group, compared to the other groups of guinea pigs bone marrow micronucleus rate with the control group (2.46 ‰) were significantly improved, but there is no obvious pattern in each set of test results; 73 days after treatment, the guinea pig bone marrow micronucleus rate has not decreased to the normal levels. This suggests that the nano-materials caused genetic damage in guinea pigs. ⑤ in chromosome film, you can see more debris and particles in the bone marrow cells, which is the result of chromosomal breakage and missing, reflects the rare earth nano material guinea pig chromosome causing greater damage. In addition, depending on the route of exposure of guinea pigs take different tissue blocks obtained by HE staining.

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CLC: > Medicine, health > Basic Medical > Medical science in general > Biomedical Engineering > General issues > Biomaterial
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