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Systematic Analysis of Huangqi Seeds and Its Adulterants

Author: ZuoChong
Tutor: NieFengDie
School: Hebei Medical University
Course: Pharmaceutical Analysis
Keywords: Astragalus Seed Chromosome PAGE Protein RAPD DNA Microscopic characteristics Scanning electron microscopy Supermicro characteristics Thin layer chromatography UV spectrophotometry
CLC: R282.5
Type: Master's thesis
Year: 2005
Downloads: 124
Quote: 0
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Abstract


The dried root of Astragalus is the legume mongholicus or film Astragalus , the cultivated mongholicus qi solid form drugs, quality is good . Now the herbs comes mainly from the scale planted , so the quality of Astragalus provenance Astragalus yield and quality prerequisites and directly guarantee . Often adulterants flat stem astragalus and alfalfa seed in the seed market currently flooding the market. In order to prevent misuse of pseudo- varieties of the Son , and site-specific selection of authentic Astragalus provenance Appearance of the experiment from the positive pseudo varieties sub microscopic characteristics , physical and chemical properties as well as from the molecular level of two genuine Seed and its adulterants the identification of research . Both deeper level of basic research , there are fast , simple , practical way to meet primary needs . Traits , microscopic , physical and chemical identification purposes : Application is fast and easy, practical way to build two genuine Seed and its two common adulterants the flat stems astragalus and alfalfa seed Appearance , microscopic characteristics, physical and chemical the comprehensive identification of the nature of information indications. Method: 1 . Observed Appearance . Decolorization surface sheet prepared : ( 1) decolorization : dial the take the kinds of Peel and cut into 1-2 mm small pieces immersed chloral hydrate ( 1:1 ) solution . (2 ) Compression observed. Paraffin transverse slice preparation : ( 1 ) drawn and fixed : seeds the FAA were fixed more than two weeks . (2) Dehydration: washed successively with a concentration of 70 % , 80%, 90 % , 95% ethanol solution , anhydrous ethanol for dehydration , the solution was exchanged at intervals of 2-3 h . ( 3 ) transparent : 1-2 h after soaking xylene - ethanol ( 1:1 ) , transferred to xylene transparent to seed date . (4 ) dip wax :

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CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Chinese herbal medicines > Identification of medicinal materials
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