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At present, foreign marine tunicate ascidians physiologically active substance research is in rapid development stage, but on the sheath from Ascidian sac or sheath body extracted efficient antitumor drugs has not been reported. Chinese scholars Ascidian research is extremely rare , with only relatively preliminary exploratory research , the problem is not related to physical activity . The rich resources of the ocean sea squirts , especially the coastal waters of Shandong Province, sea squirts resources is extremely rich, compared with other countries , not only did not get developed , but also greatly plagued the development of fishing and sailing , and should therefore be cause for great attention. In this paper, the following aspects of physiologically active substances Ascidian conducted a preliminary study . First , the handle ascidian capsule sheath and the sheath body for fine peeling of the material to avoid mutual interference , a better positioning of material . Ascidian body of the sheath , according to the current extraction of natural products commonly used separation method , the sample is freeze-dried , washed with methanol : toluene ( 3:1 ) soaked , collecting the filtrate by distillation under reduced pressure to obtain a concentrated aqueous solution was extracted with 1M NaNO3 , aqueous phase was collected , concentrated under reduced pressure to precipitate crystals NaNO3 . NaNO3 crystals soaked again with chloroform , chloroform extraction was collected , concentrated under reduced pressure to give red-brown crude extract was shaped sheath body . Crude extract was washed with 5% NaHCO3, Na2CO3, NaOH extraction grading , and three kinds of extracts were concentrated, and freeze-dried , denoted P Ⅰ, P Ⅱ, P Ⅲ. Wherein , P Ⅰ, P Ⅱ by polyamide column chromatography, eluting with H2O, 10% CH3CH2OH, 50% CH3CH2OH, 100% CH3CH2OH 4 of gradient elution order ; 4 concentrate and then the gradient SephadexLH-20 gel chromatography, high semi-preparative liquid chromatography analysis and , ultimately, six kinds of components : F Ⅰ A, F Ⅰ B, F Ⅲ A, F Ⅲ B, Fw and F Ⅳ.
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