Dissertation > Excellent graduate degree dissertation topics show

Study on the Interaction between Serum Albumin、Drugs and New Fluorescence Probes with ICT Behavior

Author: PangYueHong
Tutor: ShuangShaoMin；Man Shing Wong
School: Shanxi University
Course: Pharmaceutical Analysis
Keywords: Human serum albumin Bendroflumethiazide Intramolecular charge transfer Hydrochlorothiazide 4 - Diphenyl -4' -di- phenyl - boric acid (DBBA) - Diphenyl phenyl boric acid ( a DBPA ) Fluorescent probe
CLC: R96
Type: Master's thesis
Year: 2005
Downloads: 133
Quote: 3
Read: Download Dissertation

Abstract

Chapter: This chapter provides a brief overview of the research progress of the basic structure of the protein nature, protein and drug interactions, intramolecular charge transfer studies and to describe the protein quantitative analysis of the status quo. Chapter II (a): This chapter fluorometry, UV-visible spectrophotometry bendroflumethiazide human serum protein interactions, as well as in a physiological buffer solution, the temperature, the presence of metal ions on the binding constants bendroflumethiazide human serum protein interaction studies from a thermodynamic point of view, derived thermodynamic parameters △ H and △ S, △ H = 49.28KJ mol ^{-1 gt; 0, △ S = 258.83J mol ^{-1 K ^{the -1 gt; basis for F (?) x {9864} ster non-radiative energy transfer mechanism obtained a combination of both position r = 1.47. The experiments show that the bendrofluazide static quenching process strong quenching effect on the fluorescence of HSA, bendroflumethiazide and protein binding constants and the temperature has little effect on the binding constant. The presence of metal ions is reduced, generally 30% -55% reduction in binding constants. Bendroflumethiazide with human serum albumin subdomain Ⅱ A hydrophobic interactions between. Chapter II (b): This chapter fluorometry, UV-visible spectrophotometry different acidity, the the antihypertensive diuretic hydrochlorothiazide (Hydrochlorothiazide) with human serum albumin (HSA) interaction between different temperatures. Obtain the drugs with others under different acidity to Serum Albumin formation constant, to discuss the impact of trace metal ion formation constant of drugs and serum albumin, and determined according to the thermodynamic constant force between the drug and serum albumin type, on this basis, based on Foster F (?) x {9864} ster non-radiative energy transfer mechanism hydrochlorothiazide and human serum albumin combined with each other to the distance between the donor - acceptor energy transfer efficiency. Chapter 3: This synthesis novel borate compound 4 - Diphenyl-4'-diphenyl-borate (DBBA), under ambient conditions, the use of ultraviolet-visible absorption spectrum and fluorescence spectra of this compound in different polarities The luminescence properties of the solvent in the fluorescence quantum yield, fluorescence lifetime, the difference of the dipole moment were characterized. The experiment showed that the fluorescence spectra of compounds DBBA solvent effect. Calculated according to the method of Lippert-Mataga compound DBBA in the dipole moments of the difference in the excitation around 5.0D. This compound in different polar solvents in the fluorescence quantum yield, fluorescence lifetime is small, and the emission spectra of red shift, dipole distance difference increases, thus further indicates that the compound has the intramolecular charge transfer characteristics. Chapter 4: UV-visible absorption and fluorescence spectra of newly synthesized compounds: - diphenyl}}}

Study on the Interaction between Serum Albumin、Drugs and New Fluorescence Probes with ICT Behavior

Abstract

Related Dissertations