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The purpose of lung ischemia-reperfusion injury cardiopulmonary bypass and lung transplantation postoperative respiratory dysfunction or failure, ischemic postconditioning ischemia-reperfusion injury has a protective effect on the heart and liver, the study was designed to investigate the in vivo case processing mechanism of the protective effects of lung ischemia-reperfusion injury, and may take some more reasonable reflow clinical pulmonary vascular occlusion after ischemia provides a theoretical basis. Materials and Methods 24 healthy SD rats, female, weighing 280-320mg, 3% pentobarbital sodium (30 mg / kg) by intraperitoneal injection of anesthesia, tracheotomy an endotracheal tube connected to a small animal ventilator control breathing , respiratory rate 60 times / min, inspiratory to expiratory ratio of 1:1.5, work pressure (tidal volume) 0.02MP. Enter the chest through left 5th intercostal space, free left hilar over the blocking with. The animals were randomly divided into 3 groups (n = 8). Sham operation group (SO group): infusion 105 min; control group (IR group): ischemia 45min, reperfusion 60min; brief reperfusion treatment group (IPC group) after ischemia: ischemia 45min 1min ischemia 1min, repeated five times, and then the full restoration of perfusion 50 min animals were killed after the end of the test, remove the left lung, the lung tissue SOD, MDA and MPO and lung wet / dry weight ratio (W / D) detection and organization Morphological observation. The resulting data were expressed as mean ± standard deviation (X ± S) said that the experimental data using one-way ANOVA, all at the completion of the SPSS 10.0 statistical software, P lt; 0.05 as statistically significant difference standard. Results SOD, MPO and MDA content of lung tissue change lung ischemia-reperfusion, compared with the SO group, the IR lung tissue MDA content and MPO activity was significantly higher (P lt; 0.01), and SOD activity was significantly decreased (P lt; 0.01); compared with IR group and IPC group lung tissue MDA decreased (P lt; 0.05), MPO activity decreased significantly (P lt; 0.01), and SOD activity was significantly higher (P lt; 0.01 ); compared with the SO group, IPC group and IR group, lung tissue MDA content and MPO activity increased (P lt; 0.05), SOD activity was significantly decreased (P lt; 0.01). Lung tissue W / D changes in IR group was significantly higher than the SO group, IPC group was significantly lower than that of the IR group, the differences were statistically significant (P lt; 0.01). Morphological examination of lung tissue reperfusion after 60min, IR lung tissue visible mild lung congestion, lung, trachea little foamy discharge, clear to see SO group structure in the light microscope, the alveolar wall is thin, IR group significantly widened alveolar septa, significant edema, a large number of inflammatory cell infiltration, alveolar space of red blood cells, inflammatory cells and a large number of slurry; the above lesions IPC group was significantly lighter than the IR group. The conclusion 1.IPC can reduce vascular endothelial injury caused by the increased pulmonary capillary permeability. 2 can reduce the infiltration of neutrophils in ischemic lung tissue. 3 can inhibit lipid peroxidation, reducing oxygen free after reperfusion
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