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Folate is the body's normal development of important micro-nutrients, which relates to the synthesis of dUMP to dTMP, homocysteine ??(HC) simultaneously through the synthesis of methionine (Met), S-adenosyl methionine (SAM) of the biochemical processes that affect DNA A Residues. Therefore, folic acid, vitamin B 12 ( 12 ) lack of Met / SAM synthesis is blocked, can cause the dUMP accumulated and incorporated into DNA, leading to a variety of DNA structural damage; can affect the cells, particularly the DNA methylation process, which affects gene expression and chromosome segregation. In this study, cytoplasmic blocked micronucleus assay (CBMN) study of folic acid on the maintenance of patients with breast cancer and their role of lymphocyte genomic stability control individuals and differences, and to explore the important elements in the folic acid metabolic pathways Met B 12 lack of genotoxic effects BRCAI mutations in breast cancer patients with lymphoblastic cell line and normal human lymphoblastic cell line. Study from 24 patients with breast cancer, 30 cases of normal human peripheral blood lymphocytes were separated, containing 30nM 120nM be 240 nM folic acid RPMI-1640 medium (8% dialyzed fetal calf serum, 100U double anti 2mM glutamyl eight days blocking cytokinesis with cytochalasin B (CB) amide, pH7.0) culture, the cells were harvested 28 hours after genotoxic effects of folate deficiency on human lymphocytes CMBN analysis: To confirm the Met, B 12 folate metabolism and genetic stability, test 15,50 μM Met with 75 ~ 1200 pM B 12 combination of 12 kinds of RPMI-1640 medium ( 8% dialyzed fetal calf serum, 100U double-antibody, 2 mM glutamine, pH 7.0) and training to carry BRCAI, gene mutations in patients with breast cancer, lymphoblastic cell line GM13705 and normal human lymphoblastoid cell line GM12593, eight days the cells relaxin B (CB) blocked cytokinesis, cells were harvested after 28 hours, the Met, B 12 CMBN analysis of the impact of the human lymphoblastoid cell line genomic stability. The results showed that: 1, the test concentration range, folate concentrations in patients with breast cancer and their control individual lymphocyte genetic damage significantly negatively related (r = -0.248 - 0.832, p lt; 0.0001): folic acid in 30nM, genetic damage were significantly higher than 120nM and 240nM (p lt; 0.001 ~ 0.05): 120nM and 240nM between the two test groups were not observed significant difference between each indicator, when folic acid rose 120nM minimize genetic damage, suggesting that under the conditions of this experiment, 120nM folic acid to prevent human lymphocytes genetic damage
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