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Effect of Arsenic Trioxide On Primary Acute Lymphoblastic Leukemia Cells

Author: LiWuPing
Tutor: TangAiPing
School: Jiangxi Medical College
Course: Internal Medicine
Keywords: As2O3 Acute lymphoblastic leukemia Apoptosis Caspase-3 Bcl-2
CLC: R733.71
Type: Master's thesis
Year: 2005
Downloads: 56
Quote: 0
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Abstract


Objective: To investigate arsenic trioxide (As 2 O 3 ) ( hereinafter referred to as : ALL cells ) acute lymphoblastic leukemia ( ALL ) primary leukemia cells and its mechanism for As 2 O 3 in acute lymphoblastic leukemia provide experimental basis . Methods: methyl tetrazolium salt ( MTT ) and trypan blue exclusion staining assay As 2 < / sub > the O 3 < / sub > ALL cell growth inhibition ; cell morphology observed As 2 O 3 ALL cells in vitro induced apoptosis and DNA agarose gel electrophoresis ; detected by flow cytometry ALL cells in As < Sub 2 < / sub > the O 3 < / sub > Caspase-3 activity levels before and after treatment , and Bcl-2 expression level , to explore the mechanism of apoptosis , further analysis of its clinical significance . Results : in 1 0.5 micromol / L AS 2 < / sub > O 3 < / sub > ALL cells had no effect on the growth and concentration of 1.0 - 10.0μmol / L of As of < / sub> O 3 can inhibit the growth of ALL cells in a time- and concentration-dependent manner . A ② 1.0 - 10.0μmol in / L of As the 2 < / sub > O 3 < / sub > role after 48h , ALL cells showed typical apoptosis morphological changes , DNA ladder electrophoresis \bands and flow cytometry apoptosis peak . ③ 16 cases of ALL patients with primary leukemia cells Caspase-3 activity level was 4.11 ± 1.93 ; 2.0 mmol / L As a 2 < / sub > O 3 < / sub > role 48h, Caspase-3 activity levels to 26.39 ± 8.36 , 11.54 ± 2.78 , between the two groups was significantly higher difference was statistically significant ( P lt ; 0.001 ) . ( 4 ) 16 patients with clinical treatment in 9 cases, 7 cases ineffective ; effective group ALL cells Caspase-3 activity levels to 4.83 ± 0.98 , 3.19 ± 2.50 between the two groups was no significant difference ( P GT ineffective group ; 0.05 ) 2 < / sub > O ( 5 ) by 2.0 mmol / L as 3 < / sub > role after 48 hours , the treatment of effective group ALL cells Caspase-3 activity levels up to 29.67 ± 7.93 , no

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CLC: > Medicine, health > Oncology > Hematopoietic and lymphoid neoplasms > Leukemia > Acute leukemia
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