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Detection and Analysis of Donor Stem Cells after Limbal Stem Cell Transplantation in New-Zealand Rabbits

Author: XiaoWeiBao
Tutor: ZhuangMingZhong；XuGuoXing
School: Fujian Medical
Course: Ophthalmology
Keywords: Limbal stem cell transplantation Immunohistochemistry HE staining Detect Analysis
CLC: R779.65
Type: Master's thesis
Year: 2005
Downloads: 52
Quote: 0
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Abstract


Purpose : In this study, by studying different periods of New Zealand white rabbits (New-Zealand rabbit) limbal stem cell transplantation (limbal stem cell transplantation, LSCT) for stem cell 's survival situation and proliferation differentiation status , to explore the effect of limbal stem cell transplantation , and further compare the self- body transplant and allogeneic transplantation of effect as well as immune inhibitors (immunodepressant) allograft role for clinical corneal limbal stem cell transplantation effect provide appropriate theoretical foundation and the necessary surgery means the certificate , and thus a clear effect of transplantation , objective evaluation of this value of entries surgery . Methods: 40 New Zealand white rabbits were randomly divided into four groups , each of 10 male and female mismatch eye did not have any disorders . When sub autograft group ( autograft group ) , allograft group ( allograft group ) , allograft the immunosuppressant group and the control group (control group) of 4 groups . The limbal epithelial remove build limbal stem cell defects in animal models , autologous and allogeneic transplant , in which a group of allograft use 1% cyclosporine A ( cyclosporine A , CsA) . After 1 week , 2 weeks , 1 month, 2 months and March ear vein air embolism were sacrificed 2 , take the the corneal limbus full layer corneal specimens , fixed in 10% formalin (formalin) 24 hours tissue dehydration , embedded in paraffin , by visual observation , HE staining ( Hematoxylin and eosinstaining HE) and immunohistochemistry (immunohistochemistry) 4 group effect of transplantation . Results: autologous transplantation one week after the donor stem cells are still confined to the receptor limbal epithelial cells covering the full thickness of the cornea ; 1 month later , very little , but the level ; largely restored to normal three months after the number of stem cell reserve the level of corneal complete epithelialization . Allograft 1 week , some reduction in the number of stem cells ; 1 month after corneal inflammation and significantly reduced the number of limbal stem cell reserve ; After 3 months , the limbus basically detect less than stem cells . 1% cyclosporine A allograft group , the effect of significantly even better in the allograft group alone , the number of stem cell survival between the difference was statistically significant ( P lt; 0.01 ) .

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CLC: > Medicine, health > Ophthalmology > Eye surgery and surgery > Corneal transplant
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