Dissertation > Excellent graduate degree dissertation topics show

Experimental Study on the Effect of Substance P (SP) on the Secretive Function of Lipopolysaccharides (LPS)-Injured Endothelial Cells

Author: XiaoWenMing
Tutor: WuBoZuo；ChenXiaoDong
School: Fujian Medical
Course: Surgery
Keywords: substance P lipopolysaccharides nitric oxide synthesis nitric oxide endothelin vein endothelial cells
CLC: R543
Type: Master's thesis
Year: 2005
Downloads: 63
Quote: 0
Read: Download Dissertation

Abstract


Objective: To investigate the protective effect of substance P on thesecret1ive function of normal and lipopolysaccharide-injured Human Umbilical VeinEndothelial Cells (HUVEC).Methods: Using a cell line of HUVEC(ECV304), we measured theconcentrations of ET-1 and NO in the supernatant treated with differentconcentrations of SP or SP plus LPS. In addition, the expression of iNOS and eNOSwas analyzed by immunohistochemistry.Results: Different concentrations of SP exerted an obvious effect onstimulating eNOS expression and thus increasing NO production in HUVECs withoutaffecting the expression of iNOS and ET-1. LPS(1mg/L) was able to stimulate thesecretion of NO and ET-1, up-regulate eNOS expression within 30 minutes, whilesuppressed eNOS expression and induced iNOS expression 60 minutes after treatment.Furthermore, addition of SP could obviously attenuate the promoting effect of LPS onNO and ET-1 secretion in HUVECs, and ameliorate the long-term effect ofsuppressing eNOS expression while decreasing iNOS in HUVECs induced by LPS.Conclusion: SP stimulates eNOS expression and thus increases NO productionin HUVECs without affecting iNOS and ET-1 expression. LPS (1mg/L) can stimulateNO and ET secretion, eNOS expression in HUVEC within early 30 minutes. At 1hour after LPS treatment, eNOS expression as well as NO production is significantlyincreased. SP is supposed to has a protective effect on the LPS-injured HUVECs.

Related Dissertations

  1. Domestication of Acidithiobacillus Ferroxidans and Its Application to Bio-desulfuration of Coal,X701.3
  2. Analysis on Characteristics and Discriminant of Aroma Types of Flue-Cured Tobacco,S572
  3. Identification of Marine Sreptomyces Sp. GY-4 and Researches on Its Antimicrobial Substances,Q936
  4. Study on Heat Resistance of Chrysanthemum Floral Organ and Effects of Exogenously Nitric Oxide on Heat Resistance of Chrysanthemum,S682.11
  5. Tissue-engineered Construction and Preliminary Implantation Study of Diamond-like Carbon Films for Artificial Mechanical Valve Applications,R654.2
  6. TLR4 Dependent Signaling Pathway in Lipopolysaccharide Induced Human Lung Carcinoma Cells Proliferation,R734.2
  7. The Correlation Study between Entothelin-1 Level in Blood Plasma and Hemorrheology Changes in Primary Glaucoma and Visual Function Damage Degree.,R775
  8. Effects of Cold-adaptation Swimming on Hemorheology and Quantity of NO and NOS in Aorta of SD Rats in Acute Cold Water Stress,R87
  9. The Relationship of Serum Adiponectin、Nitric Oxide Synthase and Carotid Atherosclerosis in Patients with Acute Cerebral Infarction,R743.33
  10. Vascular endothelial growth factor on endothelial cells concentration of magnesium ions,R965
  11. MWCNTs and ZnO / SnO 2 Composites and NO gas research,TB33
  12. Studies on the Catalytic Performance of Modified Activated Carbon in the Regeneration of Fe(Ⅱ)EDTA Solution,TQ424.1
  13. Electrochemical Monitoring of Signal Molecule of Plant in Vivo,Q946
  14. NO Intervention of Carbon Disulfide Toxicity on the Reproductive Secretion Function of Hypothalamus -pituitary-testis Axis in Male Rats,R363
  15. Mechanism of Lipoxin A4 Regulating Endothelial Permeability in Human Umbilical Vein Endothelial Cells,R363
  16. New mussels bionic polyurethane materials research,TB391
  17. Inpatient substance dependence treatment barriers and sleep quality research,R749.6
  18. Clinical and Experimental Research on NO and NOS Changes in Blood of Tension-type Headache,R741
  19. The Change of Substance P (SP) in Serum, Spinal Cord and Synovium of Rabbit after Performing Femoral Nerve Blocking,R687.4
  20. Effects of Different Dosage Propofol on Serum NO and NOS Expression in Tissue by Intermittent Intraperitoneal Injection in Rats,R614
  21. The Effect of Sustained Hyperuricemia on Endothelial Function in Rats,R589.7

CLC: > Medicine, health > Internal Medicine > Heart, blood vessels ( circulatory ) disease > Vascular disease
© 2012 www.DissertationTopic.Net  Mobile