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Studies on the Flavonoids in the Leaves and Stems of Panax Notoginseng

Author: ZhangZhiXin
Tutor: SongGuanBin
School: Chongqing University
Course: Biomedical Engineering
Keywords: Stem and leaf Flavonoids Extract Microwave Hepatoma cells Growth inhibition
CLC: R284.1
Type: Master's thesis
Year: 2005
Downloads: 523
Quote: 5
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Abstract


Describes the pharmacological effects of flavonoids extracted, determination, separation and structure identification methods, the chemical composition of its research progress in traditional Chinese medicine 37, carried out a systematic study of flavonoids in the stem and leaf . (1) using ethanol as solvent extraction with Soxhlet extractor, using aluminum nitrate - sodium nitrite colorimetric determination of total flavonoids extraction of total flavonoids rate as an indicator to determine the stem and leaf flavonoids best alcohol extraction process conditions: with 70% ethanol solvent extraction 4h highest total extraction yield, the notoginseng the flavonoid content of 13.55 mg / g DW, stem content of 7.28 mg / g DW, stems and leaves contains flavonoids ingredients are different. Aluminum nitrate - sodium nitrite chromogenic stability test, between 15 to 40 minutes after the color is the best time of Panax leaves total flavonoids determination. In addition, the experimental stem and leaf flavonoid extract qualitative color reaction, initially identified flavonoids mainly flavonol glycosides. (2) the use of orthogonal test method to determine the the lye and microwave - alkaline extraction of stem and leaf total flavonoids process: alkaline extraction process of the law: for dilute alkali solution of pH = 10 solvent liquid to solid ratio of 30: 1, extraction temperature 80 ° C, extracted two times, 40 minutes each time. Microwave - alkaline extraction of stem and leaf flavonoid the optimum: pH 8, the microwave power is mid-range, solid-liquid ratio of 1g: 40ml, microwave irradiation time of 10 min. Extracted 2 times. Due to the the microwave - the lye method for extraction of total flavonoids of Panax with time-saving, high efficiency, energy-saving advantages of high extraction rate in the ethanol extraction and alkaline law, so using this method as the best extraction method. (3) with chitosan stem and leaf water extract clarification treatment, the best process: the chitosan prepared clarifying agent added in an amount of 0.9% of the volume of the extraction liquid and let stand for 2 hours, The pH value was adjusted to 6 at room temperature. And then purified with AB-8 resin, the process parameters as follows: an aqueous solution of 70% ethanol as the eluent, the elution rate of 2 BV / h, the eluent used in an amount of 5 times the amount of resin volume. (4) total flavonoids polyamide column separation, three stem and leaf flavonoid components, thin layer chromatography on silica gel, chromogenic method and the identification of the ultraviolet spectrum, combined with a related study, initially identified stem and leaf contained flavonoids are quercetin, quercetin-3-O-sugar glycosides and kaempferol the phenolic-3-O-glycosides. (5) stem and leaf total flavonoids inhibit the proliferation of human hepatoma SMMC-7721 cells, the change of SMMC-7721 cell cycle distribution, or G1 phase cell percentage decline in the S phase percentage increases, this effect was dose-dependent sex.

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CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Traditional Chinese medicine chemical > Chemical analysis and identification
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