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Objective: To investigate the local injection of VEGF super- aspect ratio arbitrarily expanded flap flap survival . Methods: 16 British species of white short-haired guinea pig , male or female , were randomly divided into two groups of eight . 24 hours before surgery back flap expansion range of hair removal with the specifications for the implementation of a dilator implantation 30ml of silicone rubber soft tissue expander in the back of the two groups of guinea pigs , preoperative determination of the PU value ( perfusion expanded flap range ) once. The third day after the expansion intracapsular injection of saline , once a day , every 6 ml of total water injection five times (including intraoperative once ) . Water injection was observed after the end of three days before the implementation of the two surgical ( flap angioplasty ) , preoperative determination PU value once again within the design flap . The flap size 8cm × 2cm rectangular pedicle in bilateral iliac spine connection , the experimental group injection 20ng/100ulVEGF working fluid from the distal flap , flap both sides at intervals of about 1cm each selected five symmetrically injection site , a total of 10 bits , each point of injection of 20ng 100ul, total 200ng; Similarly, the control group injected with phosphate buffered saline (PBS). After surgery in the first four days , 10 days , 12 days , respectively, far away , in forming the flap , nearly 1/3 do PU value determination . Were sacrificed on day 12 guinea pigs , cut a flap specimen and immediately with 4 % PFA ( poly formaldehyde) were fixed , making wax block , 4um serial sections , do the morphological changes of conventional HE staining immunohistochemistry (SP method) analysis detection of proliferating cell nuclear antigen (PCNA) and the detection of vascular endothelial cell VEGF expression and microvessel density (MVD) . The establishment of a database and the results were analyzed by ANOVA with SPSS11.5 .
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