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The Study of Antitumor Effect of Tumor-draining Lymph Node Cells from Lung Cancer Patients Induced by Different Stimuluses in Vitro and in Vivo

Author: LiQiLiang
Tutor: DanBaoEn；AiJun
School: Hebei Medical University
Course: Clinical Laboratory Science
Keywords: Tumor-draining lymph node cells Specific anti-tumor effect Dendritic cells Cytokines Apoptosis
CLC: R734.2
Type: Master's thesis
Year: 2006
Downloads: 29
Quote: 0
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Abstract


Objective: To study TDLN cells in vitro and in vivo anti- tumor effect , and explore the mechanism of action of the TDLN cells , improved TDLN cell culture method for tumor-specific biological therapy to provide new ideas . Method: 1 lung cancer patients TDLN cell suspension preparation . 2 Lung Cancer preparation of single cell suspension . 3 lung cancer cell antigen preparation . TDLN cell activation : the patient TDLN cell suspension ( 1 × 106/ml ) was added to 24-well plates ( 1 ml / hole ) , are divided into three groups , and were added to the type of stimulus . The first group to add IL - 2 ( 175U/ml ) ; second group of IL - 2 ( 175U/ml ) tag ( 50μg/ml ) ; the third group IL - 2 ( 175U/ml ) GM - CSF ( 500U / ml ) of IL - 4 ( 100u/ml ) itself tag ( 50μg/ml ) . Set to 37 ° C , 5 % CO2 incubator culture . The TDLN cells aneuploidy detection . The TDLN cells phenotype analysis : after activation of TDLN cells cultured for 14 days FITC - anti - CD83 monoclonal antibody FITC-CD3/PE-CD56 and FITC-CD4/PE-CD8 , incubated at room temperature for 30 minutes , PBS washed times and analyzed by flow cytometry . 7 cytokines : Adjust TDLN cell concentration to 2 x 105/ml , added to 24-well plates ( 1 ml / hole ) , divided into three groups , the first group of IL - 2 ( 175U/ml ) ; second group to join The third group plus of IL - 2 ( 175U/ml ) tag ( 50μg/ml ) ;

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CLC: > Medicine, health > Oncology > Respiratory system tumors > Lung tumors
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