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Nitroimidazoles with antiprotozoal and antibacterial activity, the provisions of the Ministry of Agriculture in animal food residues to zero. For the monitoring of animal food nitroimidazole drug residues, other than the standard method for the quantitative research methods, ① establish Dimetridazole (DMZ) and its metabolite hydroxy Dimetridazole (DMZOH) in edible chicken tissues residues HPLC method to study the DMZ and DMZOH elimination rule. ② establish bee in ronidazole (RNZ), metronidazole (MNZ), Dimetridazole (DMZ), ornidazole (ONZ), tinidazole (TNZ) multi-residue by HPLC. DMZOH DMZ and the residue of chicken edible tissues taken by HPLC chicken tissues (muscle, liver, kidney, fat) homogenate oscillation anhydrous sodium sulfate and methylene chloride extraction, the organic layer was concentrated and the residue was dissolved in water, hexane fat, water layer over C 18 SPE column, methanol, Hypersil ODS2 column chromatography, the mobile phase is water - methanol (86:14, v / v), 320nm UV detection. The results show that four kinds of chicken tissues was measured DMZ and DMZOH minimum detection limit and lower limit of quantification are 1.0μg/kg, add the DMZ and DMZOH tissue concentration 4.0gg/kg, 2.0μg/kg, 1.0μg/kg time in muscle average recoveries were between 56.9% ~ 76.5%; liver by 63.3% ~ 72.6% in between; kidney in 64.2% ~ 73.3% between; fat at 63.5% ~ 75.7%. Coefficient of variation less than 16%, day coefficients of variation were less than 15%. Click DMZ dose 250mg/kg.bw fed chicken feed, continuous 5d, after stopping 0,1,3,5,7,10,14,21,28 d on sampling and testing. Test results show that the liver, kidney residues than muscle, fat, and maintained for a long time. But after 14d, organizations were not detected drugs. RNZ, MNZ, DMZ, ONZ, TNZ in honey and royal jelly royal jelly by HPLC multi-residue samples dissolved in hydrochloric acid, sodium chloride precipitated impurities, hexane fat, and honey samples dissolved in dilute hydrochloric acid only sample; take acid layer plus dipotassium hydrogen phosphate 15g, extracted with methylene chloride, hydrochloric acid stripping, over SCX SPE column, 0.5moL / L dipotassium hydrogen phosphate solution (pH8.8) eluate was extracted with methylene chloride, Hypersil ODS2 chromatography column with a mobile phase of water - acetonitrile (90:10, v / v), 320nm UV detection. The results show that, honey and royal jelly RNZ, MNZ, DMZ, ONZ, TNZ minimum detection limit and lower limit of quantification are 1.0μg/kg, added to the sample RNZ, MNZ, DMZ, ONZ, TNZ concentration 4.0μg/kg, 2.0μg/kg, 1.0μg/kg when
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