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The Distribution of Gonadotropin-releasing Hormone in the Brain Stem of the Cherry Valley Duck

Author: LuoGuanZhong
Tutor: PengKeMei
School: Huazhong Agricultural University
Course: Basic Veterinary Science
Keywords: Cherry Valley Gonadotropin-releasing hormone Brainstem SABC method
CLC: S834
Type: Master's thesis
Year: 2005
Downloads: 72
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Abstract


GnRH is nervous, immune, endocrine system interconnected three important signal molecules animal reproductive process is one of the most important hormones. This test is to explore the gonadotropin-releasing hormone in Cherry Valley brainstem distribution, the choice of 15 birds Cherry Valley as test animals, the use of immunohistochemical methods for its brainstem gonadotropin-releasing hormone for positioning. Experimental animals by juvenile males, juvenile females, adult females were divided into three groups. Under normal feeding conditions drawn after feeding one week. First, the Cherry Valley anesthesia on the operating table, and use them paraformaldehyde perfusion-fixed. After perfusion with surgical instruments stripped skull and the brain, and placed in formalin fixative continue fixation. Fixed nice duck brain dehydrated, transparent, and other steps made of paraffin wax dipped blocks and cut into 5-6μm thick slices. The system uses two types of staining sections: one with hematoxylin - eosin staining to observe the brainstem nuclei of the site and morphology; Other Kind by SABC immunohistochemical staining to examine the gonadotropin-releasing hormone in The distribution of the brainstem. Experimental results show that the gonadotropin-releasing hormone immunoreactive neurons in the brainstem Cherry Valley within the major nuclei have distribution. This was further discussed on the basis of the morphology of gonadotropin-releasing hormone distribution and found Cherry Valley at different developmental stages of gonadotropin-releasing hormone expression levels are not consistent; but at the same age in males and females Cherry Valley brainstem expression levels of gonadotropin-releasing hormone was no significant difference. The trial also compared with mammals Cherry Valley brainstem distribution of gonadotropin-releasing hormone differences and found that the distribution of gonadotropin-releasing hormone species quite different. Finally, this study also speculated to morphological basis for possible new gonadotropin-releasing hormone secretory pathway, you can pass the third ventricle area directly into the third ventricle play a physiological role. Results of this study may fill in poultry GnRH neuronal positioning and gonadotropin-releasing hormone secretory pathway may be on a blank area of ??research and for the development of the basic theory of reproductive endocrinology poultry provide morphological aspects of the data, and be able to Other types and different species of gonadotropin-releasing hormone neurons morphological similarities and comparative study as a basis or reference.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Poultry > Duck
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