Dissertation > Excellent graduate degree dissertation topics show

Studies on the Transformation of B.campestris L.and B.napus L.with fad2 Gene and bar Gene

Author: XiaoGang
Tutor: GuanChunYun；ChenSheYuan；XiongXingHua
School: Hunan Agricultural University
Course: Crop Genetics and Breeding
Keywords: Cole bar gene fad2 gene Petiole Agrobacterium tumefaciens -mediated method PCR detection technology
CLC: S565.4
Type: Master's thesis
Year: 2005
Downloads: 164
Quote: 2
Read: Download Dissertation

Abstract


High oleic rapeseed breeding is an important element of rapeseed breeding . Cultivation of herbicide-resistant crops is a powerful means to solve the agricultural labor costs and environmental pollution . In this study, the root tumefaciens -mediated method bar gene ( herbicide-resistant the glufosinate gene ) and antisense fad2 of genes into Brassica napus and Brassica napus , hoping to create a high oleic acid , herbicide-resistant Brassica and Brassica campestris L. breeding material . This study made ??the following related results : 1, the bar gene ( anti - herbicide glufosinate gene ) the expression cassette ( bar cassettee ) integration to the antisense expression rape fad2 gene ( oleic acid dehydrogenase ) fragment plant the expression vector pBI121.NF to construct another plant phenotype vector pBI121.XG . Hind III digestion was confirmed by colony PCR , the vector was successfully constructed . Agrobacterium tumefaciens -mediated target gene into Brassica napus Xiang oil 15 and Brassica campestris L. the pale Valley 9-4 . Won 15 Xiang oil , transformed seedlings 5 and pale Valley 9-4 transformed seedlings 7 , the conversion rate of 0.92% and 2.52% , respectively . The PCR detection fad2 of gene fragments and the bar gene has indeed transferred to the regeneration of seedlings . Petioles and cotyledons of rapeseed Festival in vitro culture conditions regeneration rate comparison , rape cotyledons section profusion of seedlings genetic transformation system . 4 , Xiang oil 15 as the material, using the CTAB method , SDS method and sorbitol method to extract genomic DNA of rape and UV spectrophotometry , agarose gel electrophoresis method for the three methods mentioned in the genomic DNA of the quality and yield to do a comparative analysis of the results showed that the sorbitol method to extract rapeseed genomic DNA was the best . 5 compares the bar gene -specific primers , and two kinds of PCR with DNA polymerase , found that the primers B441 An Biao production pfuDNA polymerase is the best primers and PCR with the bar gene was amplified from the genomic DNA of rape DNA polymerase .

Related Dissertations

  1. 50% acetochlor water emulsion in peanut, rapeseed fields Residue Analysis and Dynamic Degradation,S481.8
  2. Rare on crop body protein , amino acids and effector impact study,S312
  3. Different planting densities and fertilizer levels on Xiangzayou 763 Yield and Quality,S565.4
  4. Copper Stress on pig manure Cu accumulation and physiological characteristics of rapeseed impact study,S565.4
  5. Rapeseed and small chickweed with Sclerotinia virulence factors interacting protein-coding genes PG Screening and Characteristics,S435.654
  6. Study on Immobilization of Co-contaminated Soils by Heavy Metal Using Clay Mineral,X53
  7. Design and Implementation of a software simulation of low phase noise crystal oscillator - based,TN752
  8. Establishment of High Frequency Regeneration System in Vitro and Transformation from Cotyledons with Petiole in Radish,S631.1
  9. Genetic Transformation of ipt Gene into Rapeseed (Brassica Napus L.) Mediated by Agrobacterium Tumefaciens,S565.4
  10. Brassica clubroot fungus pathogenic factors and their impact on the Types of Infection,S435.654
  11. Study on Allelopathy Mechanism of Natural Meadow Poisonous Plants Oxytropis Ochrocephala,S452
  12. The Regulation of Organic Acids and Inhibitors to the Growth, Physiology and Root Exudates of Rape (Brassica napus L.) under Aluminum Stress,S565.4
  13. Preliminary Screening of Waterlogging Tolerant Rapeseed Resources and Genetic Diversity Analysis Based on SSR Markers,S565.4
  14. Study on the Yield Increasing Key Technologies of High Quality Rice and Rapeseed of Hanzhong,S565.4
  15. Construction of SSR markers using the multiplex fluorescence varieties of Brassica napus DNA fingerprinting,S565.4
  16. Different fertilization , planting density on the growth and yield of oilseed rape,S565.4
  17. Study on Cultivation Techniques of Direct-Seeding Rapeseed (Brassica Napus L.) Interplanted in Rice Field,S565.4
  18. Study on Nutrient Absorption of Oilseed Rape and Characteristics Compare in Different Nutrient Efficiency Types,S565.4
  19. Study on Evolution and Affecting Factors of Rapeseed Production Distribution in Yangtze Valley,S565.4
  20. Evaluation of Boron Release Characterization of Boron Fertilizers Etibor-48 and Colemanite and Effects of Them on Crops Yield and Quality,S143.7
  21. Design on 4SY-1.8 Rape Windrower,S225

CLC: > Agricultural Sciences > Crop > Economic crops > Oil crops > Rapeseed ( Brassica )
© 2012 www.DissertationTopic.Net  Mobile