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Studies on Technology of Rapid Propagation by Tissue Culture and Effects of Water Quality and Tempareture on Growth and Development of Phalaenopsis

Author: YeXiaoQu
Tutor: YiMingFang
School: China Agricultural University
Course: Ornamental Plants and Horticulture
Keywords: Phalaenopsis Tissue culture Water quality Temperature Growth and development
CLC: S682.31
Type: Master's thesis
Year: 2005
Downloads: 610
Quote: 1
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Abstract


This study is a tissue propagation technology of three newly introduced varieties of Phalaenopsis plantlets were transplanted and the impact of environmental factors on the growth and development of the Phalaenopsis . The results are as follows : 1 medium 1/4MS 6-BA2mg / L coconut milk 10% activated carbon 0.2% , the explants taken not flowering pedicels upper axillary buds inoculated sprout fast , too fast growth , high survival rate . Day and night temperature of 25 ° C / 20 ° C , the temperature constant buds sprout fast , growth is relatively fast ; However , in the training room , temperature 20 ℃ ~ 25 ℃ cultured plantlets relatively robust in the artificial climate chamber . 2 the vaccination procedure explant shear in sterile water and added to the medium the oxidant DTT10mg / L , and can effectively reduce the browning degree of the pedicel axillary bud explants . 3 pods sterile sowing culture growth 110d green pods as the best sampling time after pollination ; induced protocorm best medium for the 1st 3g / L peptone 5g / L banana juice Hyponex 10% activated carbon 0.2% . G3 6-BA3.0mg / L 10% of banana juice NAA0.2mg / L activated carbon the 0.2% protocorm proliferation rapid propagation of the best medium ; G3 6- BA2.0mg / t banana juice 10 % NAA0.4mg / L Activated carbon 0.2% plantlet growth and rooting medium . 4 the plantlets outdoor Lianmiao when placed outdoors 7d not open the cap hardening of seedlings growth . Transplanting matrix and vegetative growth of Phalaenopsis cultivation matrix are moss . Tap water and pure water on the growth and development of Phalaenopsis insignificant, but tap water can reduce costs , improve plant tolerance . Factory production , diurnal temperature 25 ℃ ~ 18 ℃, light intensity 12000 ~ 15000Lx, low-temperature processing 30d approach the Phalaenopsis flower bud differentiation induced effects best advance flowering a month ( with daytime axillary 25 ° C / 20 ℃ treatment comparison ) , spike rate was 98.1% , the number of flowers .

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CLC: > Agricultural Sciences > Gardening > Ornamental Horticulture ( flowers and ornamental trees) > Perennial Flowers > Other Flowers > Orchids
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