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Lin is a native of China's ancient aquatic perennial flowers, perennial emergent herb. Lin not only edible, but also important ornamental flowers and herbs, with high economic value. Lotus cultivation has a long history in our country, adaptable, its research has attracted widespread attention. Biological research on the lotus, traditional research focuses on taxonomic, genetic crossbreeding and so on. Therefore, the molecular biology of lotus study has important significance. In order to carry out the lotus molecular biology research, improving previous methods, the establishment of a simple, rapid method of total RNA extraction, and then to Chinese lotus (Nelumbo nucifera) leaves and petioles connected to the total RNA as a template and the corresponding designing degenerate primers by RT-PCR technique, cloned chalcone synthase gene (chs), expansion gene (expansin) and polyphenol oxidase (PPO), etc. and lotus flower color, growth, browning and disease resistance related to several important genes and sequence characteristics were analyzed. Details are as follows: 1.RNA extraction lotus tissue rich in polysaccharides, lipids, phenols and other substances, with the general approach is more difficult to extract high-quality RNA. In this study, methods to improve their predecessors, the establishment of an efficient, simple CTAB-LiCl extraction method, fast extraction of high quality lotus tissue total RNA, and high yield, good integrity, high purity, and can be further RT -PCR and other molecular biology experiments needs. In addition, this method is also applicable to other rich in polysaccharides, lipids, and other substances of plant phenolic total RNA extraction. 2.chs gene chalcone synthase (Chalcone synthase, CHS) anthocyanins in plants such as flavonoids biosynthesis of a key enzyme expression in plants may affect the amount of change in the color of flowers. By RT-PCR method, from the Chinese lotus (Nelumbo nucifera) leaves RNA successfully cloned chs exon-2, a total of seven cloned fragment length 844bp-936bp, encoding 282-312 amino acids. And other species in GenBank CHS comparing the nucleotide sequence similarity 80% -86% amino acid sequence similarity of 85% -97%. 3 expansion expansion hormone gene cloning (Expansin) is a class exists in plant cell walls and cell wall structure can buffer the network in the sheets
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