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Inhibitory Effects of Celecoxib in Combination with Minocycline on Growth of Breast Cancer Bone Metastasis: an Experiment Study

Author: LiaoZhiChao
Tutor: CaiLin
School: Wuhan University
Course: Surgery
Keywords: Celecoxib Minocycline Breast cancer bone metastasis Angiogenesis inhibitors Matrix metalloproteinase inhibitors
CLC: R737.9
Type: Master's thesis
Year: 2005
Downloads: 88
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Abstract


Bone metastases of breast cancer accounted for 70% of distant metastases of breast cancer , is a common and serious clinical disease , can cause hypercalcemia , focal pain and pathologic fractures . Breast cancer bone metastasis mechanism has not yet been clarified , and the development of medicine has not yet been a more effective approach to the prevention and treatment of bone metastasis , tumor growth and metastasis , and angiogenesis , and tumor cells is closely related to the extracellular matrix theory has gradually been recognized. Therefore , angiogenesis inhibitors Joint matrix metalloproteinase inhibitor is considered to be a new tumor therapy ideas . We use celecoxib inhibits the role of tumor angiogenesis and minocycline inhibit matrix metalloproteinase the growth of breast cancer bone metastases in animal testing research and its role in the mechanism of the initial discussed. Objective: To establish nude mouse breast cancer bone metastasis model to explore the impact of celecoxib in combination with minocycline breast cancer bone metastasis formation and growth . Methods: 24 breast cancer bone metastasis in nude mice were randomly divided into four groups , the first group the next day to be saline , intraperitoneal injection ; second group the next day be celecoxib 30mg/kg intraperitoneal injection ; third group was treated with minocycline the prime 40mg/kg, intraperitoneal injection ; fourth group are celecoxib 30mg/kg, minocycline 40mg/kg , intraperitoneal injection , were killed after 30 days . Light microscope immunohistochemistry and image analysis system to count tumor microvessel density ( MVD ) and the detection of the tumor tissue of vascular endothelial growth factor (VEGF) and matrix metalloproteinase -9 (MMP-9) expression groups tumor tissue , tumor cell apoptosis flow cytometry . Results : 1 each treatment group, tumor growth was significantly inhibited , further enhance the anti-tumor effect of the combined treatment group , the inhibition rates were 50.47% , 53.73% , and 70.09% , respectively . MVD in each treatment group , MMP - 9 and the control group to compare significantly reduce the combined treatment group , the most obvious . Metastatic tumor cell apoptosis index for each treatment group increased, significant differences in the density of tumor cells with the control group , the most obvious and joint - group differences . Conclusion : 1 . Celecoxib and minocycline could inhibit tumor growth in nude mice breast cancer bone metastases , the combination of the two has a synergistic effect , can improve the anti-tumor effect .

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Breast tumor
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