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Cervical cancer is one of the common gynecological malignancies, has become the main killer of endangering the health of women. World Health Organization statistics show that cervical cancer is highest in the global cancer mortality for women in some developing countries, even on top, about 500,000 cases of new cases of cervical cancer globally each year, about 20 million people died cervical cancer. According to incomplete statistics, China's existing cervical cancer patients about 138,000, about 50,000 people a year die of cervical cancer. Extensive research data to show that about 80% of cervical cancer associated with four kinds of high-risk type HPV infection (16,18,31, and 45), of which about 50% of cervical cancer associated with HPV16 infection, according to molecular epidemiological data the results, HPV16 type the main cause of cervical cancer in women. However, no clinical prevention measures HPV16 infection, chemotherapy and surgical treatment of advanced cervical cancer, the effect is not ideal. Cervical cancer recurrence rate is high and the treatment cost is also high. Therefore, the development of efficient, safe and inexpensive HPV DNA vaccine immunization specific prevention and treatment of HPV infection and its cause malignant lesions has important practical significance. The aniseed literature confirmed that the human papillomavirus type 16 E7 gene is a the important transforming gene expression in cervical cancer cells. Most of the studies in the target gene is mainly concentrated on the E7 gene E7 gene itself has transforming activity, however, be directly applied to the human body has a certain degree of risk, removal of the E7 transforming activity group, retained its antigen gene vaccines have been developed on the premise . The E7 protein C-terminal zinc finger structure \terminal zinc finger structure of any one of the Cys (C58G, C61G, C91G, C94G), E7 protein can completely lost the ability of the immortalized human epithelial cells. Although DNA vaccines have many advantages, but the naked DNA vaccine does not induce strong enough to protect animals against HPV infection, high levels of the immune response, and therefore must find ways to enhance its immunogenicity. In recent years, research data shows that heat shock proteins (heat shock protein, HSP) and antigen gene fusion has the potential to enhance DNA vaccine effect HSP antigen peptide complex (derived from tumor or virus-infected cells) can induce better anti-tumor and infected immune response. HSP mediated antigen presenting having a \Based on the above principle, we will mutations (91 points C → G) zinc finger E7 genes of the structure of the wild-type E7 genes, heat shock protein 70 genes were cloned into the eukaryotic expression vector pcDNA3.1 to - plasmid construct pcd -mE7 (mutations), pcd-wE7 (wild), the pcd-HSP70 three kinds recombinant plasmid by restriction enzyme digestion and PCR successfully constructed. Compared with chimeric pcd-HPVmE7-HSP70 DNA vaccine, further study of chimeric the pcd-HPVmE7-HSP70DNA vaccine immune-enhancing effects. We use four recombinant plasmid, empty vector and PBS
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