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Genetic Analysis and Mapping of Rice (Oryza Satival L.) msp1-4 and OsFH5 Mutant

Author: WangYing
Tutor: WangYouFangï¼›ZhangDaBing
School: East China Normal University
Course: Botany
Keywords: rice (Oryza sativa L.) mutants male-sterile cytoskeleton gene-mapping
CLC: S511
Type: Master's thesis
Year: 2006
Downloads: 67
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Abstract


In order to research the molecular mechanism of rice anther development and plant cytoskeleton by using gene-mapping ,RT-PCR and so on, we obtained two mutants: mspl-4 and Osfh5 ,which were treated with 60C0 ?-Ray.A rice male-sterile mutant mspl-4 with japonica cultivar 9522 background, was obtained in M3 population treated with 60C0 ?-Ray. Genetic analysis indicated that the male-sterile phenotype was controlled by a single recessive locus. To map this locus, an F2 population was constructed from the cross between the mspl-4 (japonica) and LongTeFu B (indica). This locus was mapped between the two InDel markers, WY4 and WY8, with physical distance of 247 kb. A deletion with 10 base pairs between 260bp and 269bp in MSPl open reading frame was confirmed by sequence analysis, and this led to pre-termination of MSPl translation. Phenotype analysis of mspl-4 indicated that it was similar to the msp1 mutant. To insight the expression change of rice anther developmental genes in this mutant, we carried on semi-quantitative RT-PCR analysis, and results showed that the expression level of rice UDT1 and GAMYB were reduced in mspl-4, implying that UDT1 and GAMYB are possibly the downstream genes of MSPl gene in rice pollen development.The other rice mutant Osfh5 with japonica cultivar 9522 background, was obtained in M3 population treated with 60Co ?-Ray too. Genetic analysis indicated that the mutant phenotype was controlled by a single recessive locus. To map this locus, an F2 population was constructed from the cross between the Osfh5 (japonica) and GuangLuAi 4(indica). This locus was mapped between the two InDel markers, WY706-5 and WY706-33, with physical distance of 15 kb. A deletion with 4 base pairs and a conversion with 1 base pair in eleventh extron of OsFH5 gene was confirmed by sequence analysis, and this led to pre-termination of OsFH5 translation. These results were important to know more about the function of OsFH5 gene in rice to us.

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CLC: > Agricultural Sciences > Crop > Cereal crops > Rice
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