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The EFfect of L-crnitine on the Oxidative Stress of Exercise and Energy Metabolism Enzymes of Endurance-Training Mice after Exhaustive Exercise

Author: WangQiuLing
Tutor: DingShuZhe；ChenCaiZhen
School: East China Normal University
Course: Human Movement Science
Keywords: L - carnitine Endurance Training Oxidative stress CPT-I Citrate synthase
CLC: G804.2
Type: Master's thesis
Year: 2006
Downloads: 283
Quote: 3
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Abstract


L-carnitine is the mitochondrial membrane transport of long-chain fatty acids into the mitochondria only carrier in the regulation of energy metabolism plays an important role. The mitochondrial membrane CPT-Ⅰ catalyst as L-carnitine transporter limiting enzyme of long chain fatty acids, its activity can determine the long-chain fatty acids into the mitochondria for β-oxidation of fatty acids, so that CPT-Ⅰ in the process of aerobic energy supply play a key role. The activity of CPT-Ⅰ regulation by many factors, which can make use carnitine levels is an important factor in the regulation. Exhaustive exercise, the muscles of free L-carnitine levels will decline, energy metabolism of fatty acids is restricted, can not provide the energy required for the body movement. As the movement time, the body of free radicals and cleared homeostasis will be broken, the occurrence of oxidative stress exercise and lipid peroxidation of mitochondrial damage. L-carnitine supplementation can increase muscle L-carnitine levels can be used to improve CPT Ⅰ mRNA expression levels, and promote long-chain fatty acid transport into the mitochondria, the β-oxidation of acetyl coenzyme A into the citric acid cycle, improve the regeneration of ATP into and utilization, and enhance the ability of athletes, delay exercise fatigue generated. In the field of sports medicine, there are already a lot of L-carnitine supplementation can improve antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH -Px) activity, decreased MDA content of research, and has been demonstrated in skeletal muscle endurance training can improve the M-CPT Ⅰ mRNA expression, but the current study is not yet related to L-carnitine supplementation during exercise on bone M-CPT Ⅰ muscle mRNA expression of changes. In this study, Kunming male mice by giving supplemental L-carnitine (4w), observe carnitine supplementation and endurance training on a one-time swimming in mice after exhaustive exercise antioxidant enzyme activity, and the use of reverse transcription-polymerase chain reaction (RT-PCR) technology, research and supplement L-carnitine on long-chain fatty acid carrier acetyl carnitine transferase Ⅰ (CPT Ⅰ) and the Krebs cycle rate-limiting enzyme citrate synthase mRNA expression, trying to molecular biological level to clarify the supplement L-carnitine in vivo antioxidant mechanisms, so as to provide the proper use of L-carnitine theoretical guidance. The results showed that L-carnitine supplementation can improve antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activity, decreased MDA content, while CPT Ⅰ expression and citric acid also significantly improved, so it can be inferred L-carnitine can increase ATP production, and has antioxidant, can ease the movement of oxidative stress damage to the body. Conclusions (1) L-carnitine supplementation can reduce weight gain in mice, indicating that L-carnitine supplementation can increase the oxidation of fatty acids in the body for energy. (2) L-carnitine supplementation can significantly prolong time to exhaustion disposable swim. (3) supplement L-carnitine to mouse skeletal muscle after exhaustive swimming exercise SOD, CAT and GSH-Pox activity increased significantly, MDA was significantly decreased, suggesting that L-carnitine supplementation can alleviate lipid peroxidation to reduce the production of free radicals, thereby preventing and mitigating the damage of free radicals on the body. The endurance training also allows mouse skeletal muscle after exhaustive swimming exercise SOD, CAT and GSH-Px activity increased significantly, MDA levels were significantly reduced, table

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