|
Fume is a mixed contaminants , degradation of the harmful components in the soot filter out the targeted degradation of soot lipids hexanal different strains Pseudomonas Sp.ZD-2 of Pseudomonas sp.ZD - 3 , and then mixed hanging film processing fume exhaust . A lipase production strains isolated from serious soot pollution in soil Degradation of lipids pollutants , the fermentation process was optimized by statistical experimental design methods . Plackett-Burman design experiments to examine the influence lipase fermentation carbon sources, nitrogen sources , inorganic salts , the pH , temperature and speed . Found that soy flour , K 2 HPO 4 and shaking speed lipase production influence the larger , the relative importance of 98.9% , 96.2% and 96.2% . Reuse of the center point in the design of experiments in the surface response method , in the above three factors as the independent variable , as a response to the enzyme activity of the fermentation broth , and is finally obtained by fitting the response surface functions of a lipase fermentation : y = 507.12 84.42x1 145.34x2-7.36x3-31.33x 1 2 -156.43x 2 2 0.02x 3 < / sub> 2 -16.00x1x2 0.08X1X3 0.76X2X3 extremum of the function under certain constraints , to get the maximum yield of lipase fermentation conditions : soybean meal added amount 1.32% K the 2 HPO 4 amount of 0.688% , and the shaking speed 120r/min . Mixed bacteria linked to the film 15 days after the determination of the temperature, inlet concentration , gas flow and humidity fumes degradation ability . The results showed that : a diameter of 250mm, the packing height of 300 mm , the size of 20mm × 20mm × 10mm Indus pieces of wood to a packed column of the filler , the degradation of the optimum temperature was 30 ° C , the optimum moisture content of 60% . Under this condition , when the inlet concentration of less than 380mg the / m 3 , gas flow less than 0.6m in 2 / h , the degradation rate of more than 90% . Stable at 91% in the biological degradation of the packed column under the conditions of a long-running ability . In order to test the processing performance of the actual operation of the equipment , the design of a pilot equipment and install 5 canteens in Zhejiang University . By measuring the processing efficiency of the device on the hood of 76.5% .
|