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Research on Epi-fluorescence Detection Technique for Microfluidic Chips

Author: ZhuZuo
Tutor: FanShiFuï¼›XiaoSongShan
School: Tianjin University
Course: Biomedical Engineering
Keywords: Microfluidic Chips Drop firing fluorescence detection Optical trap Data acquisition
CLC: Q503
Type: Master's thesis
Year: 2005
Downloads: 129
Quote: 2
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Abstract


Microfluidic chip (Microfluidic Chip) technology has been obtained in the field of biochemical analysis of the successful applications , such as DNA sequencing , protein and single-cell separation and detection . It is an efficient , fast , sample less reliable results the potential advantages compared with traditional capillary electrophoresis . Over the years, the study of microfluidic chip detection technology has been a focus of scientific and technological research and application of microfluidic chip and difficult . This topic is mainly engaged in the study of microfluidic chip detection technology , designed a microfluidic chip detection can be applied to fluorescence detection device , and the device used for the detection of the fluorescent dye rhodamine B and fluorescein . Experimental results show that : the system has a certain sensitivity , good linearity and repeatability . Thesis research work mainly includes the following aspects: ( 1 ) According to the information at home and abroad , analyzes the factors that affect the overall performance of the microfluidic chip detection system to construct a set of epi decline fluidic chip fluorescence detection system , and in the light path design the unique structure of the optical trap . The design and production of a data acquisition and processing system of the fluorescence detection system through the microscope objective collected the fluorescence signal in the microchannel , and then received by the photodiode , and converted into an electrical signal , and finally through sampling, processing, and data acquisition , transmission and real-time display function . Were experimental validation work : to build detection system for the detection of the fluorescent dye rhodamine B and fluorescein , to reach the detection limit , and linearity , and verify that the structure of the optical trap to a certain extent reduce the background noise . Work of this thesis, the design of the microfluidic chip detection system , making do exploration is proposed based on the decline of the fluidic chip fluorescence detection optical trap epi ideas , design and production of optics, electronics and software systems , experiments show that this work the microfluidic chip detection technology is valuable .

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CLC: > Biological Sciences > Biochemistry > General issues > Biochemical techniques
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