Dissertation > Excellent graduate degree dissertation topics show

Determination of Three Pesticide Residue by Capillary Electrophoresis with Online Concentration and Fluorescent Spectrophotometry

Author: HouFangFei
Tutor: ChenGuanHua
School: Agricultural University of Hebei
Course: Agricultural Products Processing and Storage
Keywords: Chlordimeform Electrostacking Omethoate Pushbroom Amitraz Remain
CLC: TS207.53
Type: Master's thesis
Year: 2006
Downloads: 133
Quote: 0
Read: Download Dissertation

Abstract


The electric accumulation technology is the use of capillary electrophoresis CZE mode to achieve the sample column enrichment technology, the the concentrated analyte electrophoretic velocity changes. The sweeping technique samples using capillary electrophoresis MECC mode to achieve column enrichment technology analyte in the quasi-stationary phase distribution concentrated. Both can improve the capillary electrophoresis detection limit. Capillary electrophoresis Electrostacking technology achieve the enrichment of chlordimeform online determination of chlordimeform residues in honey using capillary electrophoresis method affect the peak height of the component buffer pH, phosphate concentration , the methanol concentration, separation voltage, the methanol concentration in the sample, and injection time were optimized. Optimized measurement conditions: the concentration of 50.0mmol / L of NaH 2 PO 4 running buffer, a pH value of 6.12, the concentration of methanol in the buffer solution was 50%, the separation voltage 10KV, 80% methanol concentration in the sample solution, sample injection time of 40s. The above established under the optimum separation conditions, the peak concentration of the components of chlordimeform enrichment factor of up to 1810 times the detection limit as low as 1.12 x 10 -3 mg / kg, and the calibration curve The linear range of 2.2 ~ 11μg / L between. The method has been used to determination of chlordimeform content in commercially available honey samples spiked recovery test, the recovery rate of 95.5% to 103.0%, the result is reliable. Realized by capillary electrophoresis line sweeping technique omethoate line preconcentration new method for the determination of capillary electrophoresis detection of cabbage in the residual omethoate, buffer pH affect component peak height, boric acid the methanol concentration in the salt concentration, surfactant concentration, buffer, separation voltage and the methanol concentration in the sample as well as the injection time and the injection voltage are optimized. Optimized measurement conditions were as follows: a concentration of 80.0 mmol / L borax running buffer, pH value of 10.22, a methanol concentration of 10% in the buffer solution, the surfactant concentration of 6.0mmol / L, the separation voltage of 7KV, the sample solution The methanol concentration of 80%, and the sample injection voltage the 7KV, injection time for the 120s. The above established under the optimum separation conditions, the enrichment factor of up to 270 times the peak concentration of omethoate components detection limit can be as low as 4.5 × 10 -4 mg / kg, calibration curve The linear range of 0.18 ~ 18mg / L. The method has been applied commercially cabbage samples omethoate Determination spiked recovery test, the final determination of the recovery rate of between 90.2% to 94.7%. Amitraz through a two-step hydrolysis of 2,4 - dimethyl aniline can be generated having a strongly fluorescent substance, whereby principle, a fluorescence analysis method of measuring the residual amitraz. Of amitraz the detection limit to 1.44μg / L, the linear dynamic range was 3.59 ~ 1174μg / L, The samples spiked recoveries of 82.7% to 86.3%. The method has been used for the Determination of Amitraz residues in pears.

Related Dissertations

  1. 50% acetochlor water emulsion in peanut, rapeseed fields Residue Analysis and Dynamic Degradation,S481.8
  2. Pharmacokinetics and Residues of Florfenicol in Yellow Catfish (Pelteobagrus Fulvidraco),S948
  3. Behavior and Effect of Avermectin in Paddy Field,X592
  4. Effects of the Melamine with Cyanuric Acid on the Health and Residues in Laying Hens,S859.84
  5. Study of Effects of Votatile Phenols on Physiological Indices of Carassius Auratus and the Detection and Degradation of Votatile Phenols,X835
  6. The Study on Electrokinetic Flow Analysis System and Green Analytical Methodologies of Capillary Electrophoresis,O657.8
  7. Three pesticides in tobacco and tobacco soil dynamics and mechanism of degradation,X592
  8. Model-Based Control of Singularly Perturbed Networked Systems with Uncertain Nonlinear Disturbance,O231
  9. Class of finite groups isomorphic to keep some of the results from,O152.1
  10. Detection of Virus DsRNA in Edible Fungi from Sichuan and Dtermination of Their Sensitivity to Carbendazim,S436.46
  11. Study on Determination of Veterinary Drug Residues in Food Using HPLC and HPLC-MS,S859.84
  12. Effect of Foliage Application of Uniconazole on the Yield and Quality of Alism a Plantago-aquatica Linn,S567.239
  13. Organophosphorus pesticide degrading bacteria isolation, identification and immobilized,X592
  14. New Methods of Determination of Some Hazardous Substances in Food by GC / MS and Hpce,TS207.3
  15. Studies on the Application of Micro/Nanofluidic Chip in Medical and Biological Analysis,O658.9
  16. Study on Residual Degradation of Chlorpyrifos and Emamectin Benzoate in Cabbage, Soil and Relative Photodegradation,X592
  17. Typical soil research organochlorine pesticide residues and their bioavailability,X592
  18. Carbendazim, triadimefon in Hangzhou peony , Atractylodes and soil residue behavior,S481.8
  19. Chlorpyrifos and other pesticides in Zhejiang Radix, chrysanthemum and Residue Dynamics of soil,S481.8
  20. Dietary melamine in eggs and egg tissue residues, elimination regularity and on the health effects of laying hens,S859.8
  21. Doxycycline under different water catfish in vivo pharmacokinetic studies with residual rule,S859.19

CLC: > Industrial Technology > Light industry,handicrafts > Food Industry > General issues > Food standards and testing > Determination of food contamination > Determination of pesticide residues
© 2012 www.DissertationTopic.Net  Mobile