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Biosensors has broad prospects for application in clinical , food , environment and other fields , has developed rapidly in recent years . In the production process of the biosensor , the fixing of the biological recognition molecule is a very important aspect . The currently reported fixing methods include adsorption method, crosslinking method , covalent bonding method , entrapment method , etc. , which the polymer embedding method is an important method of fixing . Electrochemical quartz crystal impedance analysis (EQCIA) technology to quickly measure the electro-acoustic impedance of the quartz crystal and to obtain a more comprehensive crystal resonant information dynamics of electrochemical reaction or process a variety of physical and chemical parameters and material properties , such as the surface of the electrode , which can be synchronized as low the nanogram quality change , solution glued density and modified film viscoelasticity . In this paper, on the basis of previous work in this laboratory , this technology for fixing process of the study of biological molecules in the production process of the biosensor . The main contents are as follows : 1 . Biosensors concept , composition and substance fixed method of biomolecular recognition , as well as electrochemical quartz crystal microbalance (EQCM) technology and its applications . 2 , formed by oxidation of a dopamine electrically the the polymerization film ( PFDO ) for a new method in the immobilization of glucose oxidase (GOD) . EQCIA technology field monitoring a neutral aqueous solution (PBS , pH 7.0 ) the polymerization film growth as well as the fixing process of GOD . By optimizing the concentration of the phosphate buffer solution in the polymerization solution , dopamine and GOD three produced the excellent performance PFDO-GOD/Au electrode for glucose sensor detector . Under optimized conditions , the linear range of the sensor is 0.05 ~ 9mM, and the detection limit was 3 μm (S / N = 3 ) , the response time within 15s , and good stability . In addition , we take advantage of the electrochemical and EQCIA technical estimates the the immobilized GOD effective enzyme activity and enzyme
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