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Liquid phase microextraction (LPME) is a novel sample processing technology developed in recent years . The technology is simple , high efficiency of extraction , enrichment effect , anti-interference ability and , at the same time it consumes a very small amount of organic solvent , is a new technology is an environmentally friendly sample pretreatment . The technology and high-performance liquid chromatography (HPLC) combined with extremely wide range of applications in the analysis of the field of chemistry, pharmacology, biochemistry, clinical medicine , and environmental chemistry . More systematic study on the separation and analysis of the liquid - liquid - liquid microextraction - high performance liquid chromatography technique (LLLME-HPLC) of drugs in body fluids . The main contents of the paper are as follows: 1 . Recent years used sample pretreatment methods , focusing on the liquid-phase micro- extraction technology was introduced . 2 . Applications LLLME-HPLC simultaneous determination of three plasma local anesthetic lidocaine , bupivacaine , and tetracaine . For the plasma interference factors and low concentration of local anesthetic target microextraction conditions such as stirring speed , extraction time and pH value of feed phase optimized , effective removal of interfering factors in the plasma of the target were efficient enrichment established LLLME-HPLC simultaneous detection of plasma lidocaine , bupivacaine and tetracaine highly sensitive analytical methods , and successfully applied to the use of lidocaine and bupivacaine implementation waist and epidural anesthesia patient sample analysis and detection . 3 . Applications LLLME-HPLC determination of diethylstilbestrol in urine . For diethylstilbestrol low detection sensitivity of the status quo , the enrichment factor affecting target analyte factors such as the extraction solvent , organic solvent volume , stirring rate , extraction time , the pH of feed phase and phase of NaOH concentration is optimized to achieve the urine samples of diethylstilbestrol efficient separation , enrichment and detection . 4 . Establish separate analysis of the high sensitivity of sildenafil and vardenafil in human plasma . Affecting microextraction various factors such as the extraction solvent , the solvent volume ,
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