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An important goal of the Human Genome Project is the development of automated, high-speed DNA sequencing technology. Mainly four-color fluorescent labeling of capillary electrophoresis DNA sequencing. Due to environmental noise, a dye excitation spectral overlap, electrophoresis broadening effects dye mobility drift factors, resulting in the decline in the signal-to-noise ratio and resolution of the DNA fingerprinting is not conducive to the nucleotide sort need to do the necessary processing on the original map. In this thesis, the DNA profiles processing algorithm and design the appropriate software. The main functions of the software including pretreatment of DNA profiles, four-color correction and post-processing of three parts. First, the the preprocessing software DNA profiles. Preprocessing including data segment selected baseline adjustment, noise filtering, peak identification of four modules: the data the segment select delete the preceding redundant data; the baseline baseline height adjusted so that the four-color fluorescent signal line; noise filter to remove impulse noise in the signal and white noise; peak recognition select peak data, as solving crosstalk matrix data. Secondly, the software of the DNA profiles do four-color correction. The four-color correction is an important part in the processing of DNA fingerprinting, which solves the four specific wavelengths of fluorescence intensity to the concentration of the four fluorophores conversion problems. Four dye emission spectra for the labeled DNA fragments have partially overlapping, resulting in four-color fluorescence crosstalk, four color correction needs to be done on the original DNA fingerprinting. The four-color correction lies the crosstalk matrix of solving major peak method, slope method, four-dimensional space clustering method. Software slope method and four-color spatial clustering Method crosstalk matrix, by matrix transformation DNA profiles, the elimination of the four-color fluorescence crosstalk. Finally, the software to do post-processing of DNA profiles. The post-processing including deconvolution, mobility correction, normalization of three modules: the deconvolution equivalent to the inverse process of the broadening effects, can reduce the peak width, the elimination of the overlap of the peaks, to improve the resolution of the four color peaks: mobility correction adjust the relative position of the peaks of the four colors, so that the peak spectral distribution of the four-color uniform; normalized so that the height of the peak of the four-color basically the same, improving the visual effect of a Spectrum. DNA profile processing to improve the quality of the original map of the DNA, to prepare for the follow-up sort of base, and is conducive to the realization of automation, accurate DNA sequencing. The the the independent design GelRead software can browse, processing ABI and DNA profiles of the DAT format. GelRead software processing to achieve the original DNA profiles very good results can be comparable with the ABI commercial software, to achieve the desired objectives.
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