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PrefacePorphyra belongs to rhodophyta, bangiales, bangiaceae, porphyra. Porphyra Polysaccharide is very important part of porphyra, Previous studies on biological activities of polysaccharide from P. yezoensis show that it might against the clotting , hypercholesterolemia, thrombus, phlegmasia, ulcer, and is able to against gene mutation and tumor. And it is gradually known as a kind of immunoregula-tor,it can improve immunofunction of human body,stimulate some kinds of immune cell such as NK cell and the maturation, differentiation and regeneration of B lymphocyte. It can also inhence the proliferation of lymphocyte in blood increase TNF secretion of mice spleen cell,then inhence the cell toxin effect.Porphyra polysaccharides is consist of fucose, lactose, carubinose, glucose and xylopyranose. Typical structure of porphyran contains 3,6- andydro - galac-tose and sulfate, studies show that the later relates closely with the immunoregu-lating function of the PP . In this study , we studied the effect of pp to immuno-funtion through injecting pp to mice abdomen, then detect killing activity of NK cell and proliferation of lymphycyte in spleen, and the activity of TNF from spleen cell.Materials and MethodsDried Porphyra yezoensis soaked by DW can be eliminated lipid and protein ,the water extract fluid can be freezing - dried. We obtain a kind of powder pp.72 BALB/C mice were divided into 4 groups at random , Porphyra Polysaccha-ride in different dosages (0.05g ? kg"1, 0. 25g ? kg"1, 0. 5g ? kg"1) were injected intraperitoneally into three of above groups. The left one was treated with physiological saline solution alone as controls. Through 7 days injection ,at 24h, 48h,72h after the last injection,the activity of NK cell, regeneration ability of lymphycyte were detected using certain concentration of spleen cell, and the level of TNF in the cultured supematants of spleen cells from different groups of mice were measured by the method of MTT. The result is analysed by SPSS Statistical software.ResultsThe difference of killig activity of NK cell is significant between each PP dosage and control group, p <0.01, The activity of NK cell reach its top at 48h after the last injection. 0. 25g ? kg" and 0. 5 g ? kg" group compare with 0. 05g ? kg" also shows statistic difference(P <0.01).Lymphocyte proliferation of mice is detected at 24h after the last injection, results shows significant difference between each dosage of pp group and control , P < 0. 01, and shows dose - dependent relativeship in detected pp dosage range, SI value increases with pp dosage. Results of each pp dosage detected at 48h and 72h after last injection compare with control group shows insignificant difference, P>0. 05.The level TNF of each pp dosage detected at 48h and 72h after last injection compare with control group shows significant difference P <0. 01. The top of TNF level is at 48h,then begin to decrese . 0.25g ? kg"1 and 0. 5 g ? kg"1 group compare with 0.05g ? kg"1 also shows statistic difference( P <0.01) , 0. 50g ? kg" group compare with 0. 25g ? kg" shows insignificant difference , P >0.05.DiscussionIn this study, we observed the effect of pp to immunofuntion of mice, the result is that pp can inhence the status of immune function of mice , increase thesuit is that pp can inhence the status of immune function of mice , increase the activity of NK cell and regeneration of lymphocyte , increase TNF level from cultural spleen cell . we administrated pp by injecting into mice abdomen,so pp can be better contact with mice immune system to play its immunoregulating function.
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