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The Preparation of Sinter Bone Ceramic and the Effects of the Size of the Sinter Bone Ceramic Fragments to Osteoconducing on Animal Study

Author: TianXiaoHua
Tutor: GaoXin
School: Jilin University
Course:
Keywords: bone defect sinter bone ceramic granule size Computer image analysis system IOD
CLC: R783.1
Type: Master's thesis
Year: 2006
Downloads: 55
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Abstract


Sintered bovine bone was first used by Ueno et al in 1982,as a substitutefor new bone .This material was named true bone ceramic because of itscharacteristic true bone and ceramic .It is an organized crystal of bone mineralmade by sintering bovine bone at high temperature .Sintered bovine bone is theonly biomaterial with a natural trabecular structure derived from true bone andis composed of inorganic bone material .Excellent bone formation has beenobserved in and around true bone ceramic implanted in bone marrow. Theusefulness of this material has been demonstrated in animal experiments .Since1983,it has been implanted in patients with bone tumors, fresh fracture,pseudarthrosis, and various other pathological conditions of bone .It’s not clear,however, effects of the size of the sinter bone ceramic fragments toosteoconducing. Bone powders had a mean particle size of 275.42 ± 88.51mm,which has been considered ideal for osteogenesis. However, there is not beenseen the animal study report about it. The aim of this study is measure theeffects of the size of the sinter bone ceramic fragments to osteoconducingthrough animal study.First, we proceeded the some preperiod experiments, namely the preparingof sinter bone ceramic and the research of the physics and chemistry function.The result manifestation that sinter bone ceramic:① Color is white, having thespecial fluorescence in HA;② The small beam construction of bone is completemaintained, interval porosity is 84.9% ± 0.6%;③The pore size is variable,mainly at 200 ~850 um;④ Having no nitrogen chemical element, expressingto have no the protein composition, having no antigen;⑤ Sinter bone ceramiccomposition a. X-ray diffraction showed the component of sinter bone ceramicbeing HA;b. Electronics probe test result: The CaO is 46.03%, P2O5 is 31.748%,ca/p=1 / 1.83, nearly to person bone;⑥ The anti-presses strength of bone is1.2-2.9 MPa;⑦ The anti-presses strength of 0.1-0.9mm group and 0.1-0.2mmgroup obvious stronger than other groups. Therefore, sinter bone ceramic is anideal bone engineering material, but the strength descends after burning, theproper conduct and actions accepts not the heavy area bone defection.In our country most of the tests on bone grafting materials are performed inanimal limb. However, the calvarial model has many similarities to themaxillofacial region as acceptor site. Both the calvaria and the facial bones developby membranous bone deposition and show morphological and embryologicalsimilarities. In calvarial morphogenesis and bone healing the dura plays a centralrole. So, we selected the calvarial model as bone defect model. In consideration ofthe ideal diameter of the bone grain mentioned and the Bio-oss bone powdergrain diameter that commercialize, the sinter bone ceramic fragments weredivided into four groups due to the different size :A group(0.1-0.3mm)、Bgroup(0.3-0.6 mm) 、C group(0.6-0.9mm)、D group(0.1-0.9mm),and then weretransplanted into rabbit skull respectively to observe the osteoblast results. Indue time points of 3、6、8 ,12 week respectively ,X-ray films were taken on thedefect sites and experimental animals were euthanasized ,samples underwenthistotome slicing and histological study. The data received q-test accordingly byusing SPSS10. 0, with P< 0. 01 as statistical significance.The observational method of this experiment that use the special dyeing inGomori pathology besides the normal method .The improved the dyeing methodcan dye with different tissue, such as tooth, skeleton and its conjointcircumference tissue into contrast fresh and clear and gorgeous red or green.Each appearance characteristic of the organizes and cell: Blue color in cellnucleus or blue purple;bone and calcified bone ceramic quality red;Pink colorin fiber, cellulose, nerve fiber;Cartilage, gum and net fiber organizes green, sowe can see the result ossification clear and definitely.Different degrees of osteogenesis were observed grossly at fixed timepoints. Statistical analyses on the data indicate a statistical significanceexisted between A group and B、C 、D group respectively, a statisticalsignificance existed between D group and B 、C group respectively( P < 0. 01).The physics and chemistry kind of the living creature material will decidethe ossification cell activity with the crystallography characteristic, the latter canpromote the new bone organizes to succeed formation and quantities. For boneconductibility that increases material, often from the deliquescence dynamicsconstant that increases calcium phosphate salt with surface that increases thematerial responds the activity commences, is bigger for the its deliquescence insmall grain in a size in crystalloid dynamics constant too, so, the grain can’t betoo big. Also can’t be too small certainly. Takuji et al find that smallerfragments(less than 50 um) of true bone ceramic decreased the rates of DNA andcollagen synthesis in MC3T3-E1 cells. This indicates that smaller fragmentsmay not be appropriate for cell growth and that larger fragments may be neededto stimulate osteoblast-like cells. The suppression of osteoblast function by thesmaller particles is consistent with a previous report in which an inhibitoryeffect of hydroxyapatite microcrystals on cell growth was observed inMC3T3-E1 cells. The bone in the RBM defects is only partly in direct contactwith the implant surface. The non-functional, central part of the RBM is graduallyresorbed by multinucleated osteoclast-like cells. The A group grain is small, thetransplantation thing of the center department degrade quick, so six weeksafter operation the bone defect lacks the osteoconducing of sinter boneceramic, the ossification speed reduces slow. The set of B and C are big,degrade speed slow, affect the new osseous formation. However, the D group isa mixing set, which has the excellence of big grain and small grain, degradespeed and new bone formation of the speed went with should.As a word, there were significant differences among the osteoconductivepotentials of the different sizes of sinter bone ceramic granule. This result can beused as a important consult index sign to research to manufacture the imitatebone material .

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