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Studies on the Function of ERp29 in Injury and Reconstruction of Intestinal Epithelium Cell Irradiated by γ-Ray

Author: YangZuo
Tutor: SuYongPing
School: Third Military Medical University
Course: Preventive Medicine in Military
Keywords: ERp29 RNA interference Intestinal epithrlial cell gene transfection injury and reconstruction ionizing radiation
CLC: R574
Type: Master's thesis
Year: 2006
Downloads: 55
Quote: 0
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Abstract


Intestinal epithelial cells (IECs) are the major component of the intestinal mucomembranous barrier, and play important roles in the body owing to its many kinds of functions such as absorption, immunity, and secretion, and so on. IECs are sensitive to ionizing radiation (IR), and they are one of the main targets in case of whole-body irradiation. Intestinal wound induced by large does of IR can form, thus make the intestine lose its barrier function, impose intestinal infection of endogenous bacteria, and cause mult-organ dysfunction syndrome. It is the main reason of high death rate of intestinal-type radiation disease. Ionizing irradiation can give rise to a series of biochemical events inside of the cell. Free radicals that are derived from intercellular water interact with DNA and proteins, thus either inducing or reducing gene expression, as well as proteins. These proteins are associated with many important cellular process including DNA repair, apoptosis, cell cycle control, and oxidative stress response.Some studies have showed intestinal epithelial can reconstruct to some extent after IR (non-lethal). It is possible to study the protein related to injury and reconstruction of small intestinal epithelium irradiated byγ-Ray. With the accumulating evidence in the literature that new protein are found to be implicated in radiation response, the injury by radiation will be significantly reduce.Our topic is supported by NSFC,the point is to research the mechanism underlying the radiation response of small intestine epithelial cells.we have used the comparative proteome approach to isolate the protein, ERp29, which expression was regulated by ionizing radiation in mouse .The same results are found in IEC-6 cells. Through the literature retrieval, there is no report about the function of ERp29 by ionizing radiation.Combined the known function of ERp29, we suspect that ERp29 is a gene associated with injury and reconstruction in ionizing irradiation.Therefore, it is necessary to study the function of ERp29 in injury and reconstruction of intestinal epithelium cell irradiated byγ-Ray.RNAi is the process of using specific sequences of dsRNA to knock down the

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CLC: > Medicine, health > Internal Medicine > Digestive and abdominal diseases > Bowel disease
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