Dissertation > Excellent graduate degree dissertation topics show

A Tissue Engineering Study on Using Aortic Smooth Muscle Cells to Promote the Elasticity of Dermal Substitute

Author: YuLingXiang
Tutor: ZouZhongZhi
School: First Military Medical University
Course: Learning organizations learn and Embryology
Keywords: tissue engineering skin dermal substitute aortic smooth muscle cell three-dimensional culture elastin elastic fiber Gomori’s aldehyde fuchsin staining
CLC: R329
Type: Master's thesis
Year: 2002
Downloads: 22
Quote: 0
Read: Download Dissertation


Dermal substitute is an important component of the tissue engineering skin. Up to now, there is no dermal substitute that can produce elastin and form elastic fiber in vitro. In this study, we used aortic smooth muscle cells (ASMCs) as seed cells, collagen-chitin- glycosaminoglycans (GAG) as extracellular matrix, to construct dermal substitute by three-dimensional culture, and then to transplant the dermal substitute to rats subcutaneously. The elastic fibers produced by ASMCs during the three-dimensional culture and after transplantation were examined by Gomori’ s aldehyde fuchsin staining and elastin immunohistochemistry. More over, we also carried out a comparison study of the two elastic fiber staining methods.The study of ASMCs in three-dimensional culture. After primary culture and subculture, ASMCs were mixed with collagen-chitin-GAG and cultured three-dimensionally. 98 percent of the subcultured cells were approved as ASMCs by a -Actin immunohistochemistry positive staining. After three-dimensional culture (far to two weeks), elastic fibers were detected in ASMC gels.The study of transplantation of ASMC-collagen-chitin-GAG gels to rats subcutaneously. After transplantation (far to four weeks), there were not remarkable leucocytes infiltration around the ASMC gels, indicating no transplantation rejection occurred. The host blood vessels and fibroblasts growing into the ASMC gels were observed. In gels, ASMCs increased in number and appeared the morphology as active synthesis. On the 7th day, the elastic fiber enhanced significantly than in vitro. Whereas on the 28th day, no elastic fiber was detected.The comparison of Gomori’s aldehyde fuchsin staining and elastin immunohi’stochemistry. The rat aorta and skin sections were stained by Gomori’s aldehyde fuchsin staining and elastin immunohistochemistry respectively. The elastic fibers can be stained by both methods, but the elastic membranes in aorta can only be shown by Gomori’s staining completely. These results indicate that the Gomori’s staining is more effective, simple and economical.

Related Dissertations

  1. Study on How Ovary Affects Expression Patterns of Lox Gene Family and Elastin,R711
  2. Experiment of Neonate Rats Cardiomyocytes with Atelocollagen Scaffold on the Three-Dimensional Culture,R541
  3. Alterations in Elastic Fiber Content and Expression of Fibulin-5 and Lysyl Oxidase-like 1 in Uterosacral Ligaments and Vaginal Wall of Women with Pelvic Organ Prolapse,R711.7
  4. Sduties on the Modification of Elastin via Layer-By-Layer Self-Assembly,Q51
  5. Study on Tissue Engineered Dermal Substitutes,R318.08
  6. Study on the Biological Characteristics of Rat Lung Fibroblast Seeded on ECM-Scaffold,R563
  7. Culture of Neural Stem Cells in Collagen Gel and Collagen-Chitosan Scaffolds,R318
  8. Macro-microscopic Anatomy of Anal Conjoined Longitudinal Muscle and Anal Glands,R657.1
  9. Study on the Reconstruction of Tissue-Engineered Skin with Appendages Using Dermal Papillae Cells and Keratinocytes,R751
  10. Experimental Study on the Repair of Skin Defect by Transplantation of Tissue-engineered Skin,R641
  11. Study on Biocompatibility of Collagen-Chitosan Spongy Membrane,R318.1
  12. Defect Repairing Ability of Allograft Tissue Engineering Bi-layered Skin and Evaluation of Its Immunologic Rejection,R318.0
  13. Study of the Development of Human Cerebral Artery Relative to Aneurysm,R743.33
  14. Influence of Nano-TiO2 on Structures and Properties of 4GT-PTMO Elastomers,TQ340.1
  15. Construction of in Vitro Sensitization Testing Model for Chemicals and Fungal Infected Tissue-engineered Skin,R329
  16. The claret prey arterial atherosclerosis prevention and Its Mechanism,R543.5
  17. Reconstruction of a Pigmented Tissue Engineering Skin Containing Capillary-like Network,R318.1
  18. Isolation, Cultivation of Human Fetal Sebocytes and Sweat Eccrine Gland Cells in Vitro, and the Preliminary Study on Differentiation from Human Fetal Epidermal Stem Cells to Hair Follicle, Sebaceous Gland and Eccrine Sweat Gland,R644
  19. Studies on Phase Transitions and Optical Properties of Several Organic Molecular Crystals under High Pressures,O734
  20. Reconstructed Epidermal Skin Model and Its Features,R-332
  21. The Effects of Tannic Acid Post-treatment on Glutaraldehyde-fixed Bovine Jugular Vein Conduit,R318.08

CLC: > Medicine, health > Basic Medical > Human morphology > Human histology
© 2012 www.DissertationTopic.Net  Mobile