Resistin and RBP on rat white adipose tissueObjective: To investigate the effect of recombinant rat resistin protein (rResistin) and RBP on lipolysis and endo" />
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The Resistin its intervention in the function of adipose tissue , fat cells binding polypeptides (RBP)

Author: GuZuo
Tutor: ChenRongHua;GuoXiRong
School: Nanjing Medical University
Course: Pediatrics
Keywords: Resistin resistin binding peptide white adipose tissue lipid metabolism endocrine function Resistin 3T3-L1 adipocytes GLUT4 gene
CLC: R725.8
Type: Master's thesis
Year: 2006
Downloads: 69
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Abstract


Part I: Effect of Resistin' dissertation">Resistin and RBP on rat white adipose tissueObjective: To investigate the effect of recombinant rat resistin protein (rResistin) and RBP on lipolysis and endocrine in rat white adipose tissue. Method: Trypan blue was used to determine the effective concentration of RBP. Rat adipose tissue was cultured in vitro and incubated with 30ng/ml rResistin or combined with RBP of different concentrations ( 10-12mol/l, 10-10mol/l, 10-8mol/l ) . FFAs released into medium was measured by end-point method. The levels of adiponectin and TNF-a on culture supernatant were detected by ELISA kit. The mRNA expression of adiponectin and TNF-a were evaluated by RT-PCR. Results: 1) When 10-12mol/l RBP was applied, the percent of living cells was higher and the shape was unchanged. 2) The levels of FFAs released into medium was significantly increased after 24h of exposure to 30ng/ml rResistin (P<0.05), the levels were elevated by 1.15-fold. The levels of FFAs were significantly decreased after RBP was applied (P < 0.05), but there was no difference between three concentrations (P > 0.05). 3) The level of adiponectin in medium was significantly decreased after 24h of exposure to 30ng/ml rResistin (P < 0.05), but increased after incubated with RBP with the higher concentrations. 4) The expression of

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