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Lycoris (Lycoris spp.) Primary production of the famous ornamental and medicinal plants, in recent years there is destruction of germplasm collection, preservation and breeding lagging behind the problem. Tissue culture is not only an effective method of rapid propagation, but also the germplasm conservation (cryopreservation) and the foundation of innovation. In this experiment, Lycoris radiate a representative sample of its bulbs and growing parts of the tissue culture research. The 6 groups Lycoris bulbs, tissue culture and Lycoris radiate other organs, tissue culture to explore. In addition to the original habitat protection, the establishment of germplasm nursery, ultra-low temperature (-196 ° C) to save the germplasm conservation is the most long-term stability and economic choice. Glass Cryopreservation of development in recent years because of its simple and effective, being widely used. This test uses the cryopreservation technology system by vitrification trying to build Lycoris radiate shoot tips regenerated plants, to provide a reference to the long-term preservation of Lycoris and bulb plant germplasm resources. The main results are as follows: Lycoris tissue culture research cuts and growing point of the bulbs of Lycoris radiate (Lycoris radiata) material, the use of different hormones MS medium, to study the differentiation and proliferation in vitro bulblets . The highest BA 3mg / L and NAA 1.5 mg / L of culture medium, induction rate of up to 53%, induced to small plants strong and neat, Cryopreservation of applicable bulblets more than 2,000 trees. Lycoris growing point in the three different types of callus, wherein the type of hormone-containing medium may be formed in the 2,4-D 2mg / L, BA 0.5mg / L and 3 mg / L NAA, BA 3mg / L medium, embryogenic callus and transferred to differentiation medium renewable plants, is an ideal material for cryopreservation. To Lycoris radiate the model plant Lycoris China sprengeri, white flowers Lycoris Lycoris Jiangsu, straw Lycoris aurea 6 Lycoris bulbs of different groups such as tissue culture. Aurea, other can induce sprouting or bulblets, create the conditions for further cryopreservation platform for Lycoris resources. Lycoris radiate to other organs such as leaves, filaments, anthers and seeds or immature embryo tissue culture to explore early can induce callus, seeds and young leaves regenerated plants. Lycoris radiate shoot tip glass Cryopreservation of cut diameter of 1-5 mm size Lycoris radiate shoot tip cryopreservation of glass, 2-3mm Lycoris shoot tips highest survival rate (80% - 90%). The integrity of the shoot tips an impact on the survival and regeneration rate, with root and shoot primordia shoot tip culture of the recovery at the same time the hair root sprouting, growing strong. Comparative aspects of different concentrations of sucrose and the number of days of pre-culture on the survival rate of pre-culture, the results show that sucrose pre-culture medium of 0.4 mol / L 3d, shoot tips highest survival rate of 80%; sucrose
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